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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX81622 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81622, RRID:AB_11171098
- Product name
- eNOS antibody, N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of NOS3 Antibody (N-term) (GTX81622) in HL-60 cell line lysates (35μg/lane). NOS3 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NOS3 Antibody (N-term)(GTX81622) with HepG2 cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody (N-term)(GTX81622), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NOS3 Antibody (N-term) (GTX81622) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
