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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- MAB9028 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-eNOS (S1177) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P29474- Isotype
- IgG
- Antibody clone number
- 1014F
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation.
Zhang B, Liu QH, Zhou CJ, Hu MZ, Qian HX
Experimental and therapeutic medicine 2016 Nov;12(5):3181-3188
Experimental and therapeutic medicine 2016 Nov;12(5):3181-3188
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 μM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for eNOS at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
