Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F48608 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-eNos Antibody (pS1177)
- Antibody type
- Polyclonal
- Antigen
- This phospho-eNos antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS1177 of human eNos.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the phospho-eNos antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of phospho-eNos antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of phospho-eNos antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
