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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- MA5-15559 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eNOS Monoclonal Antibody (6H2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15559 targets eNOS in indirect ELISA, IHC applications and shows reactivity with Human samples. The MA5-15559 immunogen is purified recombinant fragment of human eNOS expressed in E. Coli. MA5-15559 detects eNOS which has a predicted molecular weight of approximately 133kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6H2
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells.
Tancharoen W, Aungsuchawan S, Pothacharoen P, Markmee R, Narakornsak S, Kieodee J, Boonma N, Tasuya W
Acta histochemica 2017 Mar;119(2):113-121
Acta histochemica 2017 Mar;119(2):113-121
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HAEC (Lane 1), HUVEC (Lane 2), and HeLa (Lane 3). The blot was probed with Anti-eNOS Monoclonal Antibody (Product # MA5-15559, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 140 kDa band corresponding to eNOS was observed across the cell lines tested except in HeLa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS monoclonal antibody (Product # MA5-15559) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS monoclonal antibody (Product # MA5-15559) followed with DAB staining.
