MA5-15559

antibody from Invitrogen Antibodies

Targeting: NOS3 ECNOS, eNOS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15559

Provider

Invitrogen Antibodies

Product name

eNOS Monoclonal Antibody (6H2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15559 targets eNOS in IHC applications and shows reactivity with Human samples.

Antibody clone number

6H2

Concentration

Conc. Not Determined

References

Submitted references

Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells.

Tancharoen W, Aungsuchawan S, Pothacharoen P, Markmee R, Narakornsak S, Kieodee J, Boonma N, Tasuya W
Acta histochemica 2017 Mar;119(2):113-121

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HAEC (Lane 1), HUVEC (Lane 2), and HeLa (Lane 3). The blot was probed with Anti-eNOS Monoclonal Antibody (Product # MA5-15559, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 140 kDa band corresponding to eNOS was observed across the cell lines tested except in HeLa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS monoclonal antibody (Product # MA5-15559) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS monoclonal antibody (Product # MA5-15559) followed with DAB staining.