MA3-030

antibody from Invitrogen Antibodies

Targeting: NOS3 ECNOS, eNOS

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA3-030 - Provider product page
Provider: Invitrogen Antibodies
Product name: uNOS Monoclonal Antibody (NOS-3F7-B11 B5)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA3-030 detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, human, mouse and rat tissues. MA3-030 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and immunohistochemical experiments. By Western blot, this antibody detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS. Immunohistochemical staining of NOS with MA3-030 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present. The MA3-030 antigen is purified bovine bNOS.
Reactivity: Human, Mouse, Rat, Bovine
Host: Mouse
Isotype: IgM
Antibody clone number: NOS-3F7-B11 B5
Vial size: 200 µL
Concentration: Conc. Not Determined
Storage: -20° C, Avoid Freeze/Thaw Cycles

NOS3 protein structure - MA3-030 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of uNOS (green) in C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody (NOS-3F7-B11 B5) (Product # MA3-030) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of uNOS (green) in SK-N-MC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody (NOS-3F7-B11 B5) (Product # MA3-030) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of uNOS in SK-N-MC Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a uNOS monoclonal antibody (Product # MA3-030) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). uNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of uNOS (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody (NOS-3F7-B11 B5) (Product # MA3-030) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.