Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PB9870 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Filaggrin/FLG Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for FILAGGRIN/FLG detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. FILAGGRIN/FLG information: Molecular Weight: 435170 MW; Subcellular Localization: Cytoplasmic granule . In the stratum granulosum of the epidermis, localized within keratohyalin granules (PubMed:1429717). In granular keratinocytes and in lower corneocytes, colocalizes with calpain-1/CAPN1 (PubMed:21531719); Tissue Specificity: Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417). In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417).
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of Filaggrin expression in 22RV1 whole cell lysates (lane 1). Filaggrin at 435KD was detected using rabbit anti- Filaggrin Antigen Affinity purified polyclonal antibody (Catalog # PB9870) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image