Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B2411 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ SPP1 / Osteopontin Antibody (aa288-299) LS-B2411
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Staining (0.3 ug/ml) of Human Thymus lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by using chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SPP1 / Osteopontin antibody (0.5µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Osteopontin / SPP1 Antibody (0.1µg/ml) staining of lysates of cell line MOLT4 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.