LS-B425

antibody from LSBio

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B425

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B425, RRID:AB_2286611

Product name

PathPlus™ SPP1 / Osteopontin Antibody LS-B425

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human, Mouse, Rat, Canine, Porcine

Host

Rabbit

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.