Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [4]
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- Product number
- MA5-17180 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Osteopontin Monoclonal Antibody (7C5H12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17180 targets SPP1 in indirect ELISA, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17180 immunogen is purified recombinant fragment of human SPP1 (amino acids: 167-314) expressed in E. Coli. MA5-17180 detects SPP1 which has a predicted molecular weight of approximately 35.4kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7C5H12
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps.
Biochemical and immunohistochemical investigations on bone formation and remodelling in ovariectomised rats with tamoxifen citrate administration.
Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats.
Short-term use of resveratrol in alloplastic graft material applied with calvarial bone defects in rats.
The effect of graft application and simvastatin treatment on tibial bone defect in rats. A histological and immunohistochemical study.
The effect of graft application and allopurinol treatment on calvarial bone defect in rats1.
Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells.
Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold.
Regeneration of a goat femoral head using a tissue-specific, biphasic scaffold fabricated with CAD/CAM technology.
Restoration of critical-size defects in the rabbit mandible using porous nanohydroxyapatite-polyamide scaffolds.
Žlajpah M, Boštjančič E, Tepeš B, Zidar N
Cancers 2020 Dec 11;12(12)
Cancers 2020 Dec 11;12(12)
Biochemical and immunohistochemical investigations on bone formation and remodelling in ovariectomised rats with tamoxifen citrate administration.
Baloğlu M, Gökalp Özkorkmaz E
Folia morphologica 2019;78(4):789-797
Folia morphologica 2019;78(4):789-797
Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats.
Laçin N, İzol SB, İpek F, Tuncer MC
Acta cirurgica brasileira 2019;34(9):e201900904
Acta cirurgica brasileira 2019;34(9):e201900904
Short-term use of resveratrol in alloplastic graft material applied with calvarial bone defects in rats.
Laçin N, Deveci E
Acta cirurgica brasileira 2019 Sep 12;34(7):e201900704
Acta cirurgica brasileira 2019 Sep 12;34(7):e201900704
The effect of graft application and simvastatin treatment on tibial bone defect in rats. A histological and immunohistochemical study.
Laçin N, İzol BS, Özkorkmaz EG, Deveci B, Tuncer MC
Acta cirurgica brasileira 2019 May 6;34(4):e201900408
Acta cirurgica brasileira 2019 May 6;34(4):e201900408
The effect of graft application and allopurinol treatment on calvarial bone defect in rats1.
Laçin N, İzol BS, Özkorkmaz EG, Deveci B, Tuncer MC
Acta cirurgica brasileira 2019 Mar 18;34(3):e201900306
Acta cirurgica brasileira 2019 Mar 18;34(3):e201900306
Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells.
Vidoni C, Ferraresi A, Secomandi E, Vallino L, Gardin C, Zavan B, Mortellaro C, Isidoro C
Cell communication and signaling : CCS 2019 Aug 19;17(1):98
Cell communication and signaling : CCS 2019 Aug 19;17(1):98
Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold.
Mazzoni E, D'Agostino A, Manfrini M, Maniero S, Puozzo A, Bassi E, Marsico S, Fortini C, Trevisiol L, Patergnani S, Tognon M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Oct;31(10):4555-4565
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Oct;31(10):4555-4565
Regeneration of a goat femoral head using a tissue-specific, biphasic scaffold fabricated with CAD/CAM technology.
Ding C, Qiao Z, Jiang W, Li H, Wei J, Zhou G, Dai K
Biomaterials 2013 Sep;34(28):6706-16
Biomaterials 2013 Sep;34(28):6706-16
Restoration of critical-size defects in the rabbit mandible using porous nanohydroxyapatite-polyamide scaffolds.
Guo J, Meng Z, Chen G, Xie D, Chen Y, Wang H, Tang W, Liu L, Jing W, Long J, Guo W, Tian W
Tissue engineering. Part A 2012 Jun;18(11-12):1239-52
Tissue engineering. Part A 2012 Jun;18(11-12):1239-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SPP1 monoclonal antibody (Product # MA5-17180) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using SPP1 monoclonal antibody (Product # MA5-17180) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ( a ) Adenoma; ( b ) adenoma with epithelial misplacement--epithelial misplacement in the submucosa is marked by asterix *; ( c ) adenoma with early carcinoma; invasive carcinoma in the submucosa is marked by asterix *. HE staining, orig. magnification 4x. EPHA4: Mild positive reaction in epithelial cells in adenoma ( d ). More intensive reaction in epithelial cells and in the stroma in epithelial misplacement ( e ). Focal reaction in carcinoma cells and in the stroma of invasive carcinoma ( f ). SPON2: Positive reaction in epithelial cells and in the stroma in adenoma ( g ). Positive reaction in epithelial cells and in the stroma in epithelial misplacement ( h ). Strong reaction in carcinoma cells and positive reaction in the stroma of invasive carcinoma ( i ). SPP1: Positive reaction in epithelial cells and mild reaction in the stroma in adenoma ( j ). Positive reaction in epithelial cells and in the stroma in epithelial misplacement ( k ). Strong reaction in carcinoma cells and in the stroma of invasive carcinoma ( l ). Immunohistochemistry, orig. magnification 10x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 10 Spautin-1 prevents the expression of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem cells. HGMSCs were cultured for 21 days in control medium or in differentiation medium supplemented with 1 muM resveratrol (Diff + RV) in the absence or in the presence of spautin-1 (Sp1). At the end, cell homogenates were processed for western blotting analysis of the expression of the osteoblastic transcription factor RUNX-2 and of the osteogenic differentiation markers COL1A1, OPN and OCN. Densitometry of the specific bands (average +- S.D.) of three independent experiments is shown in the histograms
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Expression of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem Cells. Adherent HGMSCs were cultured for 21 days in control medium or in differentiation medium (Diff) supplemented or not with 1 muM resveratrol (RV). At the end, cell homogenates were processed for western blotting analysis of the expression of the osteoblastic transcription factor RUNX-2 and of the osteogenic differentiation markers COL1A1, OPN and OCN. Densitometry of the specific bands (average +- S.D.) of three independent experiments is shown in the histograms