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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-34579 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Osteopontin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human bladder tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Cell-directed aptamer therapeutic targeting for cancers including those within the central nervous system.
Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.
Wei J, Song R, Sabbagh A, Marisetty A, Shukla N, Fang D, Najem H, Ott M, Long J, Zhai L, Lesniak MS, James CD, Platanias L, Curran M, Heimberger AB
Oncoimmunology 2022;11(1):2062827
Oncoimmunology 2022;11(1):2062827
Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.
Nakayasu ES, Syed F, Tersey SA, Gritsenko MA, Mitchell HD, Chan CY, Dirice E, Turatsinze JV, Cui Y, Kulkarni RN, Eizirik DL, Qian WJ, Webb-Robertson BM, Evans-Molina C, Mirmira RG, Metz TO
Cell metabolism 2020 Feb 4;31(2):363-374.e6
Cell metabolism 2020 Feb 4;31(2):363-374.e6
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human bladder tissue lysate using a SPP1 polyclonal antibody (Product # PA5-34579) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SPP1 in human bladder tissue lysate with Osteopontin Polyclonal Antibody (Product # PA5-34579) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human bladder tissue using a SPP1 polyclonal antibody (Product # PA5-34579) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of SPP1 in human bladder tissue with Osteopontin Polyclonal Antibody (Product # PA5-34579) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of SPP1 in human bladder tissue with Osteopontin Polyclonal Antibody (Product # PA5-34579) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. A) Schema demonstrating the construction of the CpG-OPN aptamer. B) Schema demonstrating the construction of the Ncl-OPN aptamer. C) Agarose gel analysis showing the annealed final products at the correct molecular weight. D) Quantification of OPN and GAPDH mRNA after co-culture of either GL261 gliomas cells (Blue) or DC2.4 myeloid cells (Orange) with either controls (Ctrl) or the CpG-OPN or Ncl-OPN aptamers. E) Western blot analysis of OPN and actin protein in either DC2.4 myeloid cells or human U87 glioblastoma cells after co-incubation with scramble controls (Ctrl-Ctrl) or the CpG-OPN or Ncl-OPN aptamers at 200 nM. F) Western blot analysis of OPN and actin protein in mouse GL261, 344SQ and 4T1.B2b incubating with 200 nM Ctrl-Ctrl, CpG-OPN or Ncl-OPN aptamers. The ratio is the calculated amount of OPN expression relative to the control set at 1.
