25715-1-AP

antibody from Proteintech Group

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Provider product page for 25715-1-AP

Western blot

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [28]
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Validations
Western blot [1]

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Product number: 25715-1-AP - Provider product page
Provider: Proteintech Group
Product name: Osteopontin antibody
Antibody type: Polyclonal
Description: Osteopontin antibody (Cat. #25715-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SPP1 protein structure - 25715-1-AP shown in red.

Submitted references Metabolic Control During Macrophage Polarization by a Citrate-Functionalized Scaffold for Maintaining Bone Homeostasis.

Wu X, Xia Y, Dai H, Hong C, Zhao Y, Wei W, Zheng D
Advanced healthcare materials 2024 Sep;13(22):e2400770

Activation of BMP4/SMAD pathway by HIF-1α in hypoxic environment promotes osteogenic differentiation of BMSCs and leads to ectopic bone formation.

Chen C, Song C, Liu B, Wang Y, Jia J, Pang K, Wang Y, Wang P
Tissue & cell 2024 Jun;88:102376

Acellular Extrinsic Fiber Cementum Is Invariably Present in the Superficial Layer of Apical Cementum in Mouse Molar.

Iwama H, Kaku M, Thant L, Mizukoshi M, Arai M, Ono Y, Kitami K, Saito I, Uoshima K
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2024 Feb;72(2):109-120

Decoding the Intercellular Cross-Talking Between Immune Cells and Renal Innate Cells in Diabetic Kidney Disease by Bioinformatics.

Zhou M, Lu F, Jiang L, Chen C, Chen S, Geng L, Sun R, Li Q, Duan S, Zhang B, Mao H, Xing C, Yuan Y
Journal of inflammation research 2023;16:3049-3062

Inhibition of AT1R/IP3/IP3R-mediated Ca(2+) release protects against calcium oxalate crystals-induced renal oxidative stress.

Hong SY, Yang YY, Wang SG, Qin BL
Chemico-biological interactions 2023 Sep 1;382:110636

Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats.

Zhu W, Qiong D, Yanli G, Min L, Ying Z, Qiyi H, Shenping Z, Xisheng W, Hui L
BMC genomics 2023 Mar 17;24(1):127

Human Amniotic Membrane Promotes Angiogenesis in an Oxidative Stress Chronic Diabetic Murine Wound Model.

Wu M, Yu Z, Matar DY, Karvar M, Chen Z, Ng B, Aoki S, Haug V, Orgill DP, Panayi AC
Advances in wound care 2023 Jun;12(6):301-315

Contribution of calcium dysregulation to impaired coronary artery contraction in Zucker diabetic fatty rats.

Liu L, Yang H, Kuang SJ, Zhang L, Zhou MY, Zeng P, Zhang MZ, Rao F, Zhou ZL, Deng CY
Clinical and experimental pharmacology & physiology 2023 Feb;50(2):158-168

miRNA‑92a inhibits vascular smooth muscle cell phenotypic modulation and may help prevent in‑stent restenosis.

Jiang F, Zhang B, Zhang X, Zhang R, Lu Q, Shi F, Xu J, Deng L
Molecular medicine reports 2023 Feb;27(2)

Overexpression of LOX-1 in hepatocytes protects vascular smooth muscle cells from phenotype transformation and wire injury induced carotid neoatherosclerosis through ALOX15.

Zhang Q, Du G, Tong L, Guo X, Wei Y
Biochimica et biophysica acta. Molecular basis of disease 2023 Dec;1869(8):166805

Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma.

Lu H, Qian J, Cheng L, Shen Y, Chu T, Zhao C
BMJ open respiratory research 2023 Dec 11;10(1)

Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by Cobalt Chloride.

Zhou X, Luo C, Fan J, Gao G, Wang T, Zhang H, Wei A
The world journal of men's health 2023 Apr;41(2):363-372

Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β-catenin signalling.

Wang Y, Gan Y, Dong Y, Zhou J, Zhu E, Yuan H, Li X, Wang B
Journal of cellular and molecular medicine 2023 Apr;27(7):950-961

Deficient DNASE1L3 facilitates neutrophil extracellular traps-induced invasion via cyclic GMP-AMP synthase and the non-canonical NF-κB pathway in diabetic hepatocellular carcinoma.

Li N, Zheng X, Chen M, Huang L, Chen L, Huo R, Li X, Huang Y, Sun M, Mai S, Wu Z, Zhang H, Liu J, Yang CT
Clinical & translational immunology 2022;11(4):e1386

M1 macrophage-derived extracellular vesicle containing tsRNA-5006c promotes osteogenic differentiation of aortic valve interstitial cells through regulating mitophagy.

Xia H, Gao M, Chen J, Huang G, Xiang X, Wang Y, Huang Z, Li Y, Su S, Zhao Z, Zeng Q, Ruan Y
PeerJ 2022;10:e14307

Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker.

Ho CC, Wu WT, Lin YJ, Weng CY, Tsai MH, Tsai HT, Chen YC, Yet SF, Lin P
Particle and fibre toxicology 2022 Jun 23;19(1):43

Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling.

Chen M, Shan L, Gan Y, Tian L, Zhou J, Zhu E, Yuan H, Li X, Wang B
Cellular and molecular life sciences : CMLS 2022 Jan 30;79(2):107

The Preventive Effect of Systemic Honokiol and Systemic Pentoxifylline on Epidural Fibrosis.

Secinti KD, Secinti IE, Kurutas EB
Turkish neurosurgery 2021;31(6):936-943

High-mobility group AT-Hook 1 mediates the role of nuclear factor I/X in osteogenic differentiation through activating canonical Wnt signaling.

Wu X, Wang X, Shan L, Zhou J, Zhang X, Zhu E, Yuan H, Wang B
Stem cells (Dayton, Ohio) 2021 Oct;39(10):1349-1361

A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone.

Fu Y, Xin Z, Ling Z, Xie H, Xiao T, Shen X, Lin J, Xu L, Jiang H
Molecular medicine (Cambridge, Mass.) 2021 Jul 22;27(1):81

Citrate regulates extracellular matrix mineralization during osteoblast differentiation in vitro.

Wu X, Dai H, Yu S, Zhao Y, Long Y, Li W, Tu J
Journal of inorganic biochemistry 2021 Jan;214:111269

Diversified transcriptional responses of myeloid and glial cells in spinal cord injury shaped by HDAC3 activity.

Wahane S, Zhou X, Zhou X, Guo L, Friedl MS, Kluge M, Ramakrishnan A, Shen L, Friedel CC, Zhang B, Friedel RH, Zou H
Science advances 2021 Feb;7(9)

Magnesium Calcium Phosphate Cement Incorporating Citrate for Vascularized Bone Regeneration.

Wu X, Dai H, Yu S, Zhao Y, Long Y, Li W, Tu J
ACS biomaterials science & engineering 2020 Nov 9;6(11):6299-6308

Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2.

Zhou X, Wahane S, Friedl MS, Kluge M, Friedel CC, Avrampou K, Zachariou V, Guo L, Zhang B, He X, Friedel RH, Zou H
Nature neuroscience 2020 Mar;23(3):337-350

Strong Signs for a Weak Wall in Tricuspid Aortic Valve Associated Aneurysms and a Role for Osteopontin in Bicuspid Aortic Valve Associated Aneurysms.

Stern C, Scharinger B, Tuerkcan A, Nebert C, Mimler T, Baranyi U, Doppler C, Aschacher T, Andreas M, Stelzmueller ME, Ehrlich M, Graf A, Laufer G, Bernhard D, Messner B
International journal of molecular sciences 2019 Sep 26;20(19)

Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment.

Tejero R, Huang Y, Katsyv I, Kluge M, Lin JY, Tome-Garcia J, Daviaud N, Wang Y, Zhang B, Tsankova NM, Friedel CC, Zou H, Friedel RH
EBioMedicine 2019 Apr;42:252-269

Effects of nicotine on the metabolism and gene expression profile of Sprague‑Dawley rat primary osteoblasts.

Liang D, Wang KJ, Tang ZQ, Liu RH, Zeng F, Cheng MY, Lian QS, Wu HK
Molecular medicine reports 2018 Jun;17(6):8269-8281

Resveratrol Ameliorated Vascular Calcification by Regulating Sirt-1 and Nrf2.

Zhang P, Li Y, Du Y, Li G, Wang L, Zhou F
Transplantation proceedings 2016 Dec;48(10):3378-3386

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Osteopontin antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human Osteopontin. This antibody recognizes human, mouse antigen. The Osteopontin antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 25715-1-AP.pdf

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