Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA027541 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA027541, RRID:AB_10601446
- Product name
- Anti-SPP1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SPP1, Gene description: secreted phosphoprotein 1, Alternative Gene Names: BNSP, BSPI, ETA-1, OPN, Validated applications: WB, IHC, ICC, Uniprot ID: P10451, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions
Altered perivascular fibroblast activity precedes ALS disease onset.
Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5−CD44+ cells
Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Pereira A, Lipphaus A, Ergin M, Salehi S, Gehweiler D, Rudert M, Hansmann J, Herrmann M
Materials 2021;14(16):4431
Materials 2021;14(16):4431
Altered perivascular fibroblast activity precedes ALS disease onset.
Månberg A, Skene N, Sanders F, Trusohamn M, Remnestål J, Szczepińska A, Aksoylu IS, Lönnerberg P, Ebarasi L, Wouters S, Lehmann M, Olofsson J, von Gohren Antequera I, Domaniku A, De Schaepdryver M, De Vocht J, Poesen K, Uhlén M, Anink J, Mijnsbergen C, Vergunst-Bosch H, Hübers A, Kläppe U, Rodriguez-Vieitez E, Gilthorpe JD, Hedlund E, Harris RA, Aronica E, Van Damme P, Ludolph A, Veldink J, Ingre C, Nilsson P, Lewandowski SA
Nature medicine 2021 Apr;27(4):640-646
Nature medicine 2021 Apr;27(4):640-646
Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5−CD44+ cells
Fu D, Wang L, Chouairi F, Rose I, Abetov D, Miller A, Yamulla R, Schimenti J, Flesken-Nikitin A, Nikitin A
Nature Communications 2020;11(1)
Nature Communications 2020;11(1)
Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing.
Asakage M, Usui Y, Nezu N, Shimizu H, Tsubota K, Umazume K, Yamakawa N, Umezu T, Suwanai H, Kuroda M, Goto H
Journal of clinical medicine 2020 Oct 27;9(11)
Journal of clinical medicine 2020 Oct 27;9(11)
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and liver tissues using HPA027541 antibody. Corresponding SPP1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol