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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- HPA027541 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA027541, RRID:AB_10601446
- Product name
- Anti-SPP1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SPP1, Gene description: secreted phosphoprotein 1, Alternative Gene Names: BNSP, BSPI, ETA-1, OPN, Validated applications: WB, IHC, ICC, Uniprot ID: P10451, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Altered perivascular fibroblast activity precedes ALS disease onset
Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions
Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing
Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5−CD44+ cells
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Månberg A, Skene N, Sanders F, Trusohamn M, Remnestål J, Szczepińska A, Aksoylu I, Lönnerberg P, Ebarasi L, Wouters S, Lehmann M, Olofsson J, von Gohren Antequera I, Domaniku A, De Schaepdryver M, De Vocht J, Poesen K, Uhlén M, Anink J, Mijnsbergen C, Vergunst-Bosch H, Hübers A, Kläppe U, Rodriguez-Vieitez E, Gilthorpe J, Hedlund E, Harris R, Aronica E, Van Damme P, Ludolph A, Veldink J, Ingre C, Nilsson P, Lewandowski S
Nature Medicine 2021;27(4):640-646
Nature Medicine 2021;27(4):640-646
Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions
Pereira A, Lipphaus A, Ergin M, Salehi S, Gehweiler D, Rudert M, Hansmann J, Herrmann M
Materials 2021;14(16):4431
Materials 2021;14(16):4431
Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing
Asakage M, Usui Y, Nezu N, Shimizu H, Tsubota K, Umazume K, Yamakawa N, Umezu T, Suwanai H, Kuroda M, Goto H
Journal of Clinical Medicine 2020;9(11):3458
Journal of Clinical Medicine 2020;9(11):3458
Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5−CD44+ cells
Fu D, Wang L, Chouairi F, Rose I, Abetov D, Miller A, Yamulla R, Schimenti J, Flesken-Nikitin A, Nikitin A
Nature Communications 2020;11(1)
Nature Communications 2020;11(1)
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and liver tissues using HPA027541 antibody. Corresponding SPP1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
