Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC14331F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Osteopontin/OPN Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Osteopontin in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse Osteopontin.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement.
Paino F, La Noce M, Tirino V, Naddeo P, Desiderio V, Pirozzi G, De Rosa A, Laino L, Altucci L, Papaccio G
Stem cells (Dayton, Ohio) 2014 Jan;32(1):279-89
Stem cells (Dayton, Ohio) 2014 Jan;32(1):279-89
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Osteopontin/OPN in U937 Human Histiocytic Lymphoma Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Osteopontin/OPN Fluorescein-conjugated Monoclonal Antibody (Catalog # IC14331F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Intracellular Molecules.