MAB14331-100

antibody from R&D Systems

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB14331-100

Provider

R&D Systems

Product name

Human Osteopontin/OPN Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Osteopontin in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse Osteopontin.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

NP_000573.1


Isotype

IgG

Antibody clone number

223126

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients.

Hu H, Liu Z, Liu C
Oncology letters 2019 Mar;17(3):2788-2794

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Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage.

Yamaguchi Y, Shao Z, Sharif S, Du XY, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung LL
The Journal of biological chemistry 2013 Feb 1;288(5):3097-111

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Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis.

Mansfield AS, Holtan SG, Grotz TE, Allred JB, Jakub JW, Erickson LA, Markovic SN
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Apr;24(4):487-94

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Osteopontin induces AP-1-mediated secretion of urokinase-type plasminogen activator through c-Src-dependent epidermal growth factor receptor transactivation in breast cancer cells.

Das R, Mahabeleshwar GH, Kundu GC
The Journal of biological chemistry 2004 Mar 19;279(12):11051-64

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Osteopontin/OPN by Western Blot. Western blot shows lysates of human adipose (diabetes) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB14331) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Osteopontin/OPN at approximately 60 to 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Osteopontin/OPN by Simple Western^TM. Simple Western lane view shows lysates of human adipose tissue and human adipose (diabetes) tissue, loaded at 0.2 mg/mL. A specific band was detected for Osteopontin/OPN at approximately 63 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB14331). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.