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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- IC808P - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Osteopontin/OPN PE-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Osteopontin (OPN) in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human OPN is observed.
- Reactivity
- Mouse
- Host
- Goat
- Antigen sequence
Q547B5- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references The tumor suppressor menin prevents effector CD8 T-cell dysfunction by targeting mTORC1-dependent metabolic activation.
Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells.
The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis.
Suzuki J, Yamada T, Inoue K, Nabe S, Kuwahara M, Takemori N, Takemori A, Matsuda S, Kanoh M, Imai Y, Yasukawa M, Yamashita M
Nature communications 2018 Aug 17;9(1):3296
Nature communications 2018 Aug 17;9(1):3296
Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells.
Guidi N, Sacma M, Ständker L, Soller K, Marka G, Eiwen K, Weiss JM, Kirchhoff F, Weil T, Cancelas JA, Florian MC, Geiger H
The EMBO journal 2017 Apr 3;36(7):840-853
The EMBO journal 2017 Apr 3;36(7):840-853
The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis.
Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, Maruyama S, Kometani K, Kurosaki T, Ohara O, Nakayama T, Yamashita M
Nature communications 2014 Apr 2;5:3555
Nature communications 2014 Apr 2;5:3555
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Osteopontin/OPN in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either (A) untreated or (B) treated with LPS was stained with Goat Anti-Mouse Osteopontin/OPN PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC808P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
