NB600-1043-0.1ml

antibody from Novus Biologicals

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB600-1043-0.1ml

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Osteopontin/OPN Antibody

Antibody type

Polyclonal

Description

Unpurified. This recognises the full length osteopontin protein (which runs at 66 kD on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. This recognises the 32 kD MMP-cleaved C-fragment, but not the 40 kD N-terminal fragment.

Reactivity

Human, Mouse, Rat, Canine, Porcine

Host

Rabbit

Vial size

0.1 ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Used at a 1:1,000 dilution to detect Osteopontin by Western blot. In lane 2, reactivity is shown against 250 ng of human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG for detection.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Osteopontin/OPN Antibody [NB600-1043] - Used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.