LS-C744596

antibody from LSBio

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C744596

Provider

LSBio

Product name

SPP1 / Osteopontin Antibody LS-C744596

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human, Mouse, Rat, Canine, Porcine

Host

Rabbit

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.