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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab119430 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Osteopontin Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human Osteopontin Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 0.01-3 µg/mL IHC: 5-30 µg/mL Protein Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 17-287- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Osteopontin Antibody (Ab119430) - Western Blot All lanes: Osteopontin Antibody (Ab119430) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 32, 45 kDa Exposure time: 15.6 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Osteopontin Antibody (Ab119430) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL Osteopontin Antibody (Ab119430) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
