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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA1-9076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Osteopontin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, mouse and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Osteopontin in MOLT4 using a Osteopontin polyclonal antibody (Product # PA1-9076) at a concentration of 0.1 µg/mL. The cell lysate was prepared with RIPA buffer. The primary antibody was incubated for one hour and chemiluminescence was used for target detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Osteopontin in Human Placenta using a Osteopontin monoclonal antibody (Product #PA1-9076) at 5 µg/mL. The Human Placenta tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.
