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- References [4]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- GTX28448 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX28448, RRID:AB_368556
- Product name
- Osteopontin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Canine, Porcine
- Host
- Rabbit
Submitted references Human Skeletal Stem Cell Response to Multiscale Topography Induced by Large Area Electron Beam Irradiation Surface Treatment.
Prevention of breast cancer-induced osteolytic bone resorption by benzyl isothiocyanate.
Du-Huo-Ji-Sheng-Tang and its active component Ligusticum chuanxiong promote osteogenic differentiation and decrease the aging process of human mesenchymal stem cells.
Scaffold vascularization in vivo driven by primary human osteoblasts in concert with host inflammatory cells.
Goriainov V, Cook RB, Murray JW, Walker JC, Dunlop DG, Clare AT, Oreffo ROC
Frontiers in bioengineering and biotechnology 2018;6:91
Frontiers in bioengineering and biotechnology 2018;6:91
Prevention of breast cancer-induced osteolytic bone resorption by benzyl isothiocyanate.
Pore SK, Hahm ER, Latoche JD, Anderson CJ, Shuai Y, Singh SV
Carcinogenesis 2018 Feb 9;39(2):134-145
Carcinogenesis 2018 Feb 9;39(2):134-145
Du-Huo-Ji-Sheng-Tang and its active component Ligusticum chuanxiong promote osteogenic differentiation and decrease the aging process of human mesenchymal stem cells.
Wang JY, Chen WM, Wen CS, Hung SC, Chen PW, Chiu JH
Journal of ethnopharmacology 2017 Feb 23;198:64-72
Journal of ethnopharmacology 2017 Feb 23;198:64-72
Scaffold vascularization in vivo driven by primary human osteoblasts in concert with host inflammatory cells.
Ghanaati S, Unger RE, Webber MJ, Barbeck M, Orth C, Kirkpatrick JA, Booms P, Motta A, Migliaresi C, Sader RA, Kirkpatrick CJ
Biomaterials 2011 Nov;32(32):8150-60
Biomaterials 2011 Nov;32(32):8150-60
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rabbit anti-osteopontin (GTX28448) was used at a 1:1,000 dilution to detect Osteopontin by Western blot. In lane 2, reactivity is shown against 250 ng of human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) for detection.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- The osteopontin antibody (GTX28448) is used at 1:1000 dilution on a blot with 250ng human osteopontin (lane 2)and MMP-cleaved osteopontin (lane 3)
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rabbit anti-Osteopontin (GTX28448) was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required
