Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C745279 - Provider product page
- Provider
- LSBio
- Product name
- COL2A1 / Collagen II Alpha 1 Antibody LS-C745279
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Anti collagen II antibody showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.