MA5-12786

antibody from Invitrogen Antibodies

Targeting: COL2A1 AOM, SEDC, STL1

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12786

Provider

Invitrogen Antibodies

Product name

Collagen II Monoclonal Antibody (2B1.5), Biotin

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-12786 targets Collagen II in FACS, IF, IHC (P), and WB applications and shows reactivity with Bovine, Chicken, Human, mouse, and Rat samples. The MA5-12786 immunogen is a purified preparation of lathyritic type II collagen from embryonic chicken sternum.

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

2B1.5

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

PLK2 modulation of enriched TAp73 affects osteogenic differentiation and prognosis in human osteosarcoma.

Li W, Zhang X, Xi X, Li Y, Quan H, Liu S, Wu L, Wu P, Lan W, Shao Y, Li H, Chen K, Hu Z
Cancer medicine 2020 Jun;9(12):4371-4385

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Maintaining mRNA integrity during decalcification of mineralized tissues.

Belluoccio D, Rowley L, Little CB, Bateman JF
PloS one 2013;8(3):e58154

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FGF23 suppresses chondrocyte proliferation in the presence of soluble α-Klotho both in vitro and in vivo.

Kawai M, Kinoshita S, Kimoto A, Hasegawa Y, Miyagawa K, Yamazaki M, Ohata Y, Ozono K, Michigami T
The Journal of biological chemistry 2013 Jan 25;288(4):2414-27

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Quantitative polarized light microscopy of unstained mammalian cochlear sections.

Kalwani NM, Ong CA, Lysaght AC, Haward SJ, McKinley GH, Stankovic KM
Journal of biomedical optics 2013 Feb;18(2):26021

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Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy.

Rui YF, Lui PP, Wong YM, Tan Q, Chan KM
Stem cells and development 2013 Apr 1;22(7):1076-85

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Combined effect of subchondral drilling and hyaluronic acid with/without diacerein in full-thickness articular cartilage lesion in rabbits.

Suwannaloet W, Laupattarakasem W, Sukon P, Ong-Chai S, Laupattarakasem P
TheScientificWorldJournal 2012;2012:310745

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An in vitro study of collagen hydrogel to induce the chondrogenic differentiation of mesenchymal stem cells.

Zhang L, Yuan T, Guo L, Zhang X
Journal of biomedical materials research. Part A 2012 Oct;100(10):2717-25

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Vinculin functions as regulator of chondrogenesis.

Koshimizu T, Kawai M, Kondou H, Tachikawa K, Sakai N, Ozono K, Michigami T
The Journal of biological chemistry 2012 May 4;287(19):15760-75

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Heart repair by reprogramming non-myocytes with cardiac transcription factors.

Song K, Nam YJ, Luo X, Qi X, Tan W, Huang GN, Acharya A, Smith CL, Tallquist MD, Neilson EG, Hill JA, Bassel-Duby R, Olson EN
Nature 2012 May 13;485(7400):599-604

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Chondrogenesis in scaffolds with surface modification of elastin and poly-L-lysine.

Kuo YC, Chung CY
Colloids and surfaces. B, Biointerfaces 2012 May 1;93:85-91

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Cartilage regeneration by culturing chondrocytes in scaffolds grafted with TATVHL peptide.

Kuo YC, Wang CC
Colloids and surfaces. B, Biointerfaces 2012 May 1;93:235-40

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Application of albumin-grafted scaffolds to promote neocartilage formation.

Lyu SR, Kuo YC, Lin MH, Hsieh WH, Chuang CW
Colloids and surfaces. B, Biointerfaces 2012 Mar 1;91:296-301

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The molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation.

Masago Y, Hosoya A, Kawasaki K, Kawano S, Nasu A, Toguchida J, Fujita K, Nakamura H, Kondoh G, Nagata K
Journal of cell science 2012 Mar 1;125(Pt 5):1118-28

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Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH
Journal of Korean medical science 2012 Jun;27(6):586-93

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The mouse palate and its cellular responses to midpalatal suture expansion forces.

Katebi N, Kolpakova-Hart E, Lin CY, Olsen BR
Orthodontics & craniofacial research 2012 Aug;15(3):148-58

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A comprehensive characterization study of human bone marrow mscs with an emphasis on molecular and ultrastructural properties.

Karaöz E, Okçu A, Gacar G, Sağlam O, Yürüker S, Kenar H
Journal of cellular physiology 2011 May;226(5):1367-82

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Surface modification with peptide for enhancing chondrocyte adhesion and cartilage regeneration in porous scaffolds.

Kuo YC, Wang CC
Colloids and surfaces. B, Biointerfaces 2011 May 1;84(1):63-70

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Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study).

Adas G, Arikan S, Karatepe O, Kemik O, Ayhan S, Karaoz E, Kamali G, Eryasar B, Ustek D
Langenbeck's archives of surgery 2011 Jan;396(1):115-26

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Heparin-conjugated scaffolds with pore structure of inverted colloidal crystals for cartilage regeneration.

Kuo YC, Tsai YT
Colloids and surfaces. B, Biointerfaces 2011 Feb 1;82(2):616-23

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FGF-2 abolishes the chondrogenic effect of combined BMP-6 and TGF-beta in human adipose derived stem cells.

Hildner F, Peterbauer A, Wolbank S, Nürnberger S, Marlovits S, Redl H, van Griensven M, Gabriel C
Journal of biomedical materials research. Part A 2010 Sep 1;94(3):978-87

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Pancreatic islet derived stem cells can express co-stimulatory molecules of antigen-presenting cells.

Karaoz E, Okçu A, Saglam O, Genc ZS, Ayhan S, Kasap M
Transplantation proceedings 2010 Nov;42(9):3663-70

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Inverted colloidal crystal scaffolds for uniform cartilage regeneration.

Kuo YC, Tsai YT
Biomacromolecules 2010 Mar 8;11(3):731-9

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Isolation and in vitro characterisation of dental pulp stem cells from natal teeth.

Karaöz E, Doğan BN, Aksoy A, Gacar G, Akyüz S, Ayhan S, Genç ZS, Yürüker S, Duruksu G, Demircan PC, Sariboyaci AE
Histochemistry and cell biology 2010 Jan;133(1):95-112

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Tissue-engineered polyethylene oxide/chitosan scaffolds as potential substitutes for articular cartilage.

Kuo YC, Hsu YR
Journal of biomedical materials research. Part A 2009 Oct;91(1):277-87

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Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers.

Karaoz E, Aksoy A, Ayhan S, Sariboyaci AE, Kaymaz F, Kasap M
Histochemistry and cell biology 2009 Nov;132(5):533-46

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Characterization of collagenous matrix assembly in a chondrocyte model system.

Yingst S, Bloxham K, Warner LR, Brown RJ, Cole J, Kenoyer L, Knowlton WB, Oxford JT
Journal of biomedical materials research. Part A 2009 Jul;90(1):247-55

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Chondrocyte phenotype and ectopic ossification in collagenase-induced tendon degeneration.

Lui PP, Fu SC, Chan LS, Hung LK, Chan KM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Feb;57(2):91-100

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Preservation of structure and immunoreactivity at the vitreoretinal interface of the rabbit eye.

Pfeffer BA, Bernstein SA, Bartels SP
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2009 Feb;247(2):193-205

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Sox9 expression of alginate-encapsulated chondrocytes is stimulated by low cell density.

Bernstein P, Dong M, Graupner S, Corbeil D, Gelinsky M, Günther KP, Fickert S
Journal of biomedical materials research. Part A 2009 Dec;91(3):910-8

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Collagen type distribution in the healthy human tympanic membrane.

Knutsson J, Bagger-Sjöbäck D, von Unge M
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2009 Dec;30(8):1225-9

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Articular cartilage regeneration with autologous marrow aspirate and hyaluronic Acid: an experimental study in a goat model.

Saw KY, Hussin P, Loke SC, Azam M, Chen HC, Tay YG, Low S, Wallin KL, Ragavanaidu K
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 2009 Dec;25(12):1391-400

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Articular cartilage increases transition zone regeneration in bone-tendon junction healing.

Wong MW, Qin L, Lee KM, Leung KS
Clinical orthopaedics and related research 2009 Apr;467(4):1092-100

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Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells.

Kretlow JD, Jin YQ, Liu W, Zhang WJ, Hong TH, Zhou G, Baggett LS, Mikos AG, Cao Y
BMC cell biology 2008 Oct 28;9:60

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In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: influence of cell seeding density and collagen concentration.

Hui TY, Cheung KM, Cheung WL, Chan D, Chan BP
Biomaterials 2008 Aug;29(22):3201-12

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FBS suppresses TGF-beta1-induced chondrogenesis in synoviocyte pellet cultures while dexamethasone and dynamic stimuli are beneficial.

Bilgen B, Orsini E, Aaron RK, Ciombor DM
Journal of tissue engineering and regenerative medicine 2007 Nov-Dec;1(6):436-42

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A proteomic analysis of adult rat bone reveals the presence of cartilage/chondrocyte markers.

Schreiweis MA, Butler JP, Kulkarni NH, Knierman MD, Higgs RE, Halladay DL, Onyia JE, Hale JE
Journal of cellular biochemistry 2007 May 15;101(2):466-76

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Mechanical force-induced midpalatal suture remodeling in mice.

Hou B, Fukai N, Olsen BR
Bone 2007 Jun;40(6):1483-93

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Mechanical interlocking of engineered cartilage to an underlying polymeric substrate: towards a biohybrid tissue equivalent.

Romito L, Ameer GA
Annals of biomedical engineering 2006 May;34(5):737-47

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Tissue engineered cartilage on collagen and PHBV matrices.

Köse GT, Korkusuz F, Ozkul A, Soysal Y, Ozdemir T, Yildiz C, Hasirci V
Biomaterials 2005 Sep;26(25):5187-97

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Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo.

Lin Y, Luo E, Chen X, Liu L, Qiao J, Yan Z, Li Z, Tang W, Zheng X, Tian W
Journal of cellular and molecular medicine 2005 Oct-Dec;9(4):929-39

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Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo.

Lin Y, Luo E, Chen X, Liu L, Qiao J, Yan Z, Li Z, Tang W, Zheng X, Tian W
Journal of cellular and molecular medicine 2005 Oct-Dec;9(4):929-39

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Differentiation-induced loss of heparan sulfate in human exostosis derived chondrocytes.

Hecht JT, Hayes E, Haynes R, Cole WG, Long RJ, Farach-Carson MC, Carson DD
Differentiation; research in biological diversity 2005 Jun;73(5):212-21

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Galectin-3 is a downstream regulator of matrix metalloproteinase-9 function during endochondral bone formation.

Ortega N, Behonick DJ, Colnot C, Cooper DN, Werb Z
Molecular biology of the cell 2005 Jun;16(6):3028-39

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Microsurgical anatomy of the lateral walls of the pituitary fossa.

Peker S, Kurtkaya-Yapicier O, Kiliç T, Pamir MN
Acta neurochirurgica 2005 Jun;147(6):641-8; discussion 649

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Abnormalities in cartilage and bone development in the Apert syndrome FGFR2(+/S252W) mouse.

Wang Y, Xiao R, Yang F, Karim BO, Iacovelli AJ, Cai J, Lerner CP, Richtsmeier JT, Leszl JM, Hill CA, Yu K, Ornitz DM, Elisseeff J, Huso DL, Jabs EW
Development (Cambridge, England) 2005 Aug;132(15):3537-48

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Transdifferentiation of smooth muscle cells into chondrocytes in atherosclerotic arteries in situ: implications for diffuse intimal calcification.

Bobryshev YV
The Journal of pathology 2005 Apr;205(5):641-50

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Paracrine and autocrine signals promoting full chondrogenic differentiation of a mesoblastic cell line.

Locker M, Kellermann O, Boucquey M, Khun H, Huerre M, Poliard A
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2004 Jan;19(1):100-10

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Macroscopic cartilage formation with embryonic stem-cell-derived mesodermal progenitor cells.

Nakayama N, Duryea D, Manoukian R, Chow G, Han CY
Journal of cell science 2003 May 15;116(Pt 10):2015-28

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Bone defects in latent TGF-beta binding protein (Ltbp)-3 null mice; a role for Ltbp in TGF-beta presentation.

Dabovic B, Chen Y, Colarossi C, Zambuto L, Obata H, Rifkin DB
The Journal of endocrinology 2002 Oct;175(1):129-41

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Bone defects in latent TGF-beta binding protein (Ltbp)-3 null mice; a role for Ltbp in TGF-beta presentation.

Dabovic B, Chen Y, Colarossi C, Zambuto L, Obata H, Rifkin DB
The Journal of endocrinology 2002 Oct;175(1):129-41

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Growth defect in Grg5 null mice is associated with reduced Ihh signaling in growth plates.

Wang WF, Wang YG, Reginato AM, Plotkina S, Gridley T, Olsen BR
Developmental dynamics : an official publication of the American Association of Anatomists 2002 May;224(1):79-89

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Dedifferentiation of mammalian myotubes induced by msx1.

Odelberg SJ, Kollhoff A, Keating MT
Cell 2000 Dec 22;103(7):1099-109

---

Dedifferentiation of mammalian myotubes induced by msx1.

Odelberg SJ, Kollhoff A, Keating MT
Cell 2000 Dec 22;103(7):1099-109

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Collagen II using a monoclonal antibody (Product # MA5-12786).

Conjugate

Biotin

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Reprogramming non-cardiomyocytes toward a cardiac fate in vivo by GHMT a. Heart sections of Fsp1-cre/Rosa26-LacZ mice uninjured or three weeks post-MI followed by injection of GFP or GHMT retroviruses. GFP-infected myocardium showed only beta-gal + non-cardiomyocytes, GHMT-infected myocardium showed extensive beta-gal + non-cardiomyocytes and cardiomyocytes. Black boxes in top panels are enlarged in lower panels. Scale bar, 40 mum. b. cTnT immunostaining and X-gal staining of heart sections of Fsp1-cre/Rosa26-LacZ mice uninjured or three weeks post-MI followed by injection of GFP or GHMT retroviruses. The same cell is marked with the same number. Several beta-gal + cells in GHMT-infected injured hearts expressed cTnT and displayed organized sarcomere structure. White boxes are enlarged in insets. Sections of injured hearts were taken at the border zone. Scale bar, 40 mum. c. Quantification of beta-gal + cardiomyocytes in border zones and infarct zones from GFP-infected (168 sections, n=3) and GHMT-infected hearts of Fsp1-cre/Rosa26-LacZ mice (20 sections, n=2) post-MI. Sections were taken at 4 levels with an interval of 250 mum below LAD ligation site. Data are presented as mean +- std. d. Staining of border zone from GHMT-infected hearts of Fsp1-cre/Rosa26-LacZ mice post-MI by X-gal (blue), anti-cTnT (red) and anti-Cx43 (green). Gap junctions (green) were observed between beta-gal + and beta-gal - cardiomyocytes (A and B) and between beta-gal + cardiomyocytes (C and

Conjugate

Biotin