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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-13026 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Collagen II Monoclonal Antibody (6B3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-13026 targets Collagen II in IF and IHC (P) applications and shows reactivity with Amphibian, Chicken, Human, and mouse samples. The MA5-13026 immunogen is purified type II collagen; obtained from the adult chicken sternum by limited pepsinization.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6B3
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references The transplantation of particulated juvenile allograft cartilage and synovium for the repair of meniscal defect in a lapine model.
Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects.
Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking.
Connexin43 Mutant Patient-Derived Induced Pluripotent Stem Cells Exhibit Altered Differentiation Potential.
Chondrogenic induction of mesenchymal stromal/stem cells from Wharton's jelly embedded in alginate hydrogel and without added growth factor: an alternative stem cell source for cartilage tissue engineering.
Trypsin-mediated enzymatic degradation of type II collagen in the human vitreous.
Supplementation with platelet-rich plasma improves the in vitro formation of tissue-engineered cartilage with enhanced mechanical properties.
Specification of chondrocytes and cartilage tissues from embryonic stem cells.
Application of design-based stereology for estimation of absolute volume and surface area of the articular and calcified cartilage compartments of undecalcified human femoral heads.
Origins and properties of dental, thymic, and bone marrow mesenchymal cells and their stem cells.
Characterization of the annulus fibrosus-vertebral body interface: identification of new structural features.
Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes.
Structural tympanic membrane changes in secretory otitis media and cholesteatoma.
Enhanced chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in low oxygen environment micropellet cultures.
Cytokine-induced increases in ADAMTS-4 messenger RNA expression do not lead to increased aggrecanase activity in ADAMTS-5-deficient mice.
Bone morphogenetic protein 2 promotes transforming growth factor β3-induced chondrogenesis of human osteoarthritic synovium-derived stem cells.
Multilineage differentiation potential of cells isolated from the human amniotic membrane.
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
Chondrogenic and osteogenic differentiations of human bone marrow-derived mesenchymal stem cells on a nanofibrous scaffold with designed pore network.
Self-assembled collagen-human mesenchymal stem cell microspheres for regenerative medicine.
The axolotl limb: a model for bone development, regeneration and fracture healing.
Potential of dental mesenchymal cells in developing teeth.
In vitro cartilage tissue formation by Co-culture of primary and passaged chondrocytes.
Agrin is highly expressed by chondrocytes and is required for normal growth.
Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332).
Selection of cell source for ligament tissue engineering.
Tissue engineered nucleus pulposus tissue formed on a porous calcium polyphosphate substrate.
Immortalization of human articular chondrocytes and induction of their phenotype.
Stabilized autologous fibrin-chondrocyte constructs for cartilage repair in vivo.
Macroscopic cartilage formation with embryonic stem-cell-derived mesodermal progenitor cells.
Yan W, Maimaitimin M, Zhao F, Fan Y, Yang S, Li Y, Cao C, Shao Z, Liu Z, Hu X, Ao Y, Cheng J
Journal of orthopaedic translation 2022 Mar;33:72-89
Journal of orthopaedic translation 2022 Mar;33:72-89
Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects.
Li Q, Zhao F, Li Z, Duan X, Cheng J, Zhang J, Fu X, Zhang J, Shao Z, Guo Q, Hu X, Ao Y
Frontiers in cell and developmental biology 2020;8:694
Frontiers in cell and developmental biology 2020;8:694
Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking.
Kondo Y, Fu J, Wang H, Hoover C, McDaniel JM, Steet R, Patra D, Song J, Pollard L, Cathey S, Yago T, Wiley G, Macwana S, Guthridge J, McGee S, Li S, Griffin C, Furukawa K, James JA, Ruan C, McEver RP, Wierenga KJ, Gaffney PM, Xia L
JCI insight 2018 Jul 26;3(14)
JCI insight 2018 Jul 26;3(14)
Connexin43 Mutant Patient-Derived Induced Pluripotent Stem Cells Exhibit Altered Differentiation Potential.
Esseltine JL, Shao Q, Brooks C, Sampson J, Betts DH, Séguin CA, Laird DW
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2017 Jun;32(6):1368-1385
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2017 Jun;32(6):1368-1385
Chondrogenic induction of mesenchymal stromal/stem cells from Wharton's jelly embedded in alginate hydrogel and without added growth factor: an alternative stem cell source for cartilage tissue engineering.
Reppel L, Schiavi J, Charif N, Leger L, Yu H, Pinzano A, Henrionnet C, Stoltz JF, Bensoussan D, Huselstein C
Stem cell research & therapy 2015 Dec 30;6:260
Stem cell research & therapy 2015 Dec 30;6:260
Trypsin-mediated enzymatic degradation of type II collagen in the human vitreous.
van Deemter M, Kuijer R, Harm Pas H, Jacoba van der Worp R, Hooymans JM, Los LI
Molecular vision 2013;19:1591-9
Molecular vision 2013;19:1591-9
Supplementation with platelet-rich plasma improves the in vitro formation of tissue-engineered cartilage with enhanced mechanical properties.
Petrera M, De Croos JN, Iu J, Hurtig M, Kandel RA, Theodoropoulos JS
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 2013 Oct;29(10):1685-92
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 2013 Oct;29(10):1685-92
Specification of chondrocytes and cartilage tissues from embryonic stem cells.
Craft AM, Ahmed N, Rockel JS, Baht GS, Alman BA, Kandel RA, Grigoriadis AE, Keller GM
Development (Cambridge, England) 2013 Jun;140(12):2597-610
Development (Cambridge, England) 2013 Jun;140(12):2597-610
Application of design-based stereology for estimation of absolute volume and surface area of the articular and calcified cartilage compartments of undecalcified human femoral heads.
Hartlev LB, Nyengaard JR, Thomsen JS, Boel LW, Stengaard-Pedersen K, Hauge EM
Journal of microscopy 2013 Aug;251(2):133-43
Journal of microscopy 2013 Aug;251(2):133-43
Origins and properties of dental, thymic, and bone marrow mesenchymal cells and their stem cells.
Komada Y, Yamane T, Kadota D, Isono K, Takakura N, Hayashi S, Yamazaki H
PloS one 2012;7(11):e46436
PloS one 2012;7(11):e46436
Characterization of the annulus fibrosus-vertebral body interface: identification of new structural features.
Nosikova YS, Santerre JP, Grynpas M, Gibson G, Kandel RA
Journal of anatomy 2012 Dec;221(6):577-89
Journal of anatomy 2012 Dec;221(6):577-89
Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes.
Taylor DW, Ahmed N, Hayes AJ, Ferguson P, Gross AE, Caterson B, Kandel RA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2012 Aug;60(8):576-87
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2012 Aug;60(8):576-87
Structural tympanic membrane changes in secretory otitis media and cholesteatoma.
Knutsson J, Bagger-Sjöbäck D, von Unge M
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2011 Jun;32(4):596-601
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2011 Jun;32(4):596-601
Enhanced chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in low oxygen environment micropellet cultures.
Markway BD, Tan GK, Brooke G, Hudson JE, Cooper-White JJ, Doran MR
Cell transplantation 2010;19(1):29-42
Cell transplantation 2010;19(1):29-42
Cytokine-induced increases in ADAMTS-4 messenger RNA expression do not lead to increased aggrecanase activity in ADAMTS-5-deficient mice.
Rogerson FM, Chung YM, Deutscher ME, Last K, Fosang AJ
Arthritis and rheumatism 2010 Nov;62(11):3365-73
Arthritis and rheumatism 2010 Nov;62(11):3365-73
Bone morphogenetic protein 2 promotes transforming growth factor β3-induced chondrogenesis of human osteoarthritic synovium-derived stem cells.
Rui YF, DU L, Wang Y, Wang Y, Lui PP, Tang TT, Chan KM, Dai KR
Chinese medical journal 2010 Nov;123(21):3040-8
Chinese medical journal 2010 Nov;123(21):3040-8
Multilineage differentiation potential of cells isolated from the human amniotic membrane.
Díaz-Prado S, Muiños-López E, Hermida-Gómez T, Rendal-Vázquez ME, Fuentes-Boquete I, de Toro FJ, Blanco FJ
Journal of cellular biochemistry 2010 Nov 1;111(4):846-57
Journal of cellular biochemistry 2010 Nov 1;111(4):846-57
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
Nigro J, White JF, Ramshaw JA, Haylock DN, Nilsson SK, Werkmeister JA
Biomaterials 2010 Jul;31(21):5689-99
Biomaterials 2010 Jul;31(21):5689-99
Chondrogenic and osteogenic differentiations of human bone marrow-derived mesenchymal stem cells on a nanofibrous scaffold with designed pore network.
Hu J, Feng K, Liu X, Ma PX
Biomaterials 2009 Oct;30(28):5061-7
Biomaterials 2009 Oct;30(28):5061-7
Self-assembled collagen-human mesenchymal stem cell microspheres for regenerative medicine.
Chan BP, Hui TY, Yeung CW, Li J, Mo I, Chan GC
Biomaterials 2007 Nov;28(31):4652-66
Biomaterials 2007 Nov;28(31):4652-66
The axolotl limb: a model for bone development, regeneration and fracture healing.
Hutchison C, Pilote M, Roy S
Bone 2007 Jan;40(1):45-56
Bone 2007 Jan;40(1):45-56
Potential of dental mesenchymal cells in developing teeth.
Yamazaki H, Tsuneto M, Yoshino M, Yamamura K, Hayashi S
Stem cells (Dayton, Ohio) 2007 Jan;25(1):78-87
Stem cells (Dayton, Ohio) 2007 Jan;25(1):78-87
In vitro cartilage tissue formation by Co-culture of primary and passaged chondrocytes.
Gan L, Kandel RA
Tissue engineering 2007 Apr;13(4):831-42
Tissue engineering 2007 Apr;13(4):831-42
Agrin is highly expressed by chondrocytes and is required for normal growth.
Hausser HJ, Ruegg MA, Brenner RE, Ksiazek I
Histochemistry and cell biology 2007 Apr;127(4):363-74
Histochemistry and cell biology 2007 Apr;127(4):363-74
Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332).
Hashimoto J, Kariya Y, Miyazaki K
Stem cells (Dayton, Ohio) 2006 Nov;24(11):2346-54
Stem cells (Dayton, Ohio) 2006 Nov;24(11):2346-54
Selection of cell source for ligament tissue engineering.
Ge Z, Goh JC, Lee EH
Cell transplantation 2005;14(8):573-83
Cell transplantation 2005;14(8):573-83
Tissue engineered nucleus pulposus tissue formed on a porous calcium polyphosphate substrate.
Séguin CA, Grynpas MD, Pilliar RM, Waldman SD, Kandel RA
Spine 2004 Jun 15;29(12):1299-306; discussion 1306-7
Spine 2004 Jun 15;29(12):1299-306; discussion 1306-7
Immortalization of human articular chondrocytes and induction of their phenotype.
He Q, Li Q, Yang L, Xu J
Chinese medical journal 2003 Sep;116(9):1351-6
Chinese medical journal 2003 Sep;116(9):1351-6
Stabilized autologous fibrin-chondrocyte constructs for cartilage repair in vivo.
Fussenegger M, Meinhart J, Höbling W, Kullich W, Funk S, Bernatzky G
Annals of plastic surgery 2003 Nov;51(5):493-8
Annals of plastic surgery 2003 Nov;51(5):493-8
Macroscopic cartilage formation with embryonic stem-cell-derived mesodermal progenitor cells.
Nakayama N, Duryea D, Manoukian R, Chow G, Han CY
Journal of cell science 2003 May 15;116(Pt 10):2015-28
Journal of cell science 2003 May 15;116(Pt 10):2015-28
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human fetus stained with Collagen II antibody using peroxidase-conjugate and AEC chromogen. Note staining of matrix in the cartilaginous plates.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cx43 expression is localized to areas of collagen 2 deposition. Representative immunofluorescent confocal micrographs of nuclei (blue), Cx43 (green), and collagen 2 (red) at day 20 chondrogenesis in control and ODDD iPSC micromass cultures. Scale bar = 50 mum.
