R1039

antibody from Acris Antibodies GmbH

Targeting: COL2A1 AOM, SEDC, STL1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1039

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1039, RRID:AB_978459

Product name

anti Collagen type II alpha 1 chain

Antibody type

Polyclonal

Antigen

Collagen type II purified from Human knee and Bovine nasal cartilage.

Reactivity

Human, Mouse, Rat, Bovine, Sheep

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

References

Submitted references

The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures.

Jagielski M, Wolf J, Marzahn U, Völker A, Lemke M, Meier C, Ertel W, Godkin O, Arens S, Schulze-Tanzil G
International journal of molecular sciences 2014 Sep 9;15(9):15821-44

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Collagen II antibody Cat.-No R1039 (Lot 26014, 1/400, 45 min RT) showed moderate staining of in FFPE Human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Image provided courtesy of LifeSpan Biosciences, Seattle, WA