Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R1039X - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1039X, RRID:AB_978465
- Product name
- anti Collagen type II alpha 1 chain
- Antibody type
- Polyclonal
- Antigen
- Collagen type II purified from Human knee and Bovine nasal cartilage.
- Reactivity
- Human, Mouse, Rat, Bovine, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.5 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
Submitted references The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures.
Jagielski M, Wolf J, Marzahn U, Völker A, Lemke M, Meier C, Ertel W, Godkin O, Arens S, Schulze-Tanzil G
International journal of molecular sciences 2014 Sep 9;15(9):15821-44
International journal of molecular sciences 2014 Sep 9;15(9):15821-44
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Collagen II antibody Cat.-No R1039 (Lot 26014, 1/400, 45 min RT) showed moderate staining of in FFPE Human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Image provided courtesy of LifeSpan Biosciences, Seattle, WA