MA5-35215

antibody from Invitrogen Antibodies

Targeting: CDX2 CDX3

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35215

Provider

Invitrogen Antibodies

Product name

CDX2 Recombinant Rabbit Monoclonal Antibody (9E0K5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA AAANLDSAQS PGPSWPAAYG APLREDWNGY APGGAAAAAN AVAHGLNGGS

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

9E0K5

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CDX2 was performed using 70% confluent log phase LoVo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CDX2 Recombinant Rabbit Monoclonal Antibody (ARC0450) (Product # MA5-35215) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing nuclear as well as cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CDX2 in 600 μg extracts of 293T cells. Samples were precipitated with 3 μg CDX2 Monoclonal antibody (Product # MA5-35215). Western blot was performed from the immunoprecipitate using CDX2 Monoclonal antibody (Product # MA5-35215) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CDX2 in paraffin-embedded human appendix. Samples were incubated with CDX2 Monoclonal antibody (Product # MA5-35215) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal imaging (Immunohistochemistry) of CDX2 in human colon. Samples were incubated with CDX2 Monoclonal antibody (Product # MA5-35215) using a dilution of 1:100 (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CDX2 in paraffin-embedded human colon carcinoma. Samples were incubated with CDX2 Monoclonal antibody (Product # MA5-35215) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.