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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29652 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116, HCT116 nuclear extract. Predicted reactivity: Mouse (94%), Rat (97%), Pig (98%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.29 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDX2 using 30 µg of H1299 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CDX2 polyclonal antibody (Product # PA5-29652) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDX2 was performed by separating 30 µg of HCT116 whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDX2 Polyclonal Antibody (Product # PA5-29652) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CDX2 in paraformaldehyde-fixed HeLa cells using a CDX2 polyclonal antibody (Product # PA5-29652) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human normal colon, using CDX2 (Product # PA5-29652) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
