37-0500

antibody from Invitrogen Antibodies

Targeting: TSC2 LAM, PPP1R160, TSC4, tuberin

Provider product page for 37-0500

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [6]
Comments [0]
Validations
Flow cytometry [1]
Other assay [8]

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Product number: 37-0500 - Provider product page
Provider: Invitrogen Antibodies
Product name: TSC2 Monoclonal Antibody (3G9D9)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 3G9D9
Vial size: 200 µL
Concentration: 0.5 mg/mL
Storage: -20°C

TSC2 protein structure - 37-0500 shown in red.

Submitted references G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Prentzell MT, Rehbein U, Cadena Sandoval M, De Meulemeester AS, Baumeister R, Brohée L, Berdel B, Bockwoldt M, Carroll B, Chowdhury SR, von Deimling A, Demetriades C, Figlia G, Genomics England Research Consortium, de Araujo MEG, Heberle AM, Heiland I, Holzwarth B, Huber LA, Jaworski J, Kedra M, Kern K, Kopach A, Korolchuk VI, van 't Land-Kuper I, Macias M, Nellist M, Palm W, Pusch S, Ramos Pittol JM, Reil M, Reintjes A, Reuter F, Sampson JR, Scheldeman C, Siekierska A, Stefan E, Teleman AA, Thomas LE, Torres-Quesada O, Trump S, West HD, de Witte P, Woltering S, Yordanov TE, Zmorzynska J, Opitz CA, Thedieck K
Cell 2021 Feb 4;184(3):655-674.e27

The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation.

Palangat M, Anastasakis DG, Fei DL, Lindblad KE, Bradley R, Hourigan CS, Hafner M, Larson DR
Genes & development 2019 May 1;33(9-10):482-497

Death-associated protein kinase 1 promotes growth of p53-mutant cancers.

Zhao J, Zhao D, Poage GM, Mazumdar A, Zhang Y, Hill JL, Hartman ZC, Savage MI, Mills GB, Brown PH
The Journal of clinical investigation 2015 Jul 1;125(7):2707-20

Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome.

Menon S, Dibble CC, Talbott G, Hoxhaj G, Valvezan AJ, Takahashi H, Cantley LC, Manning BD
Cell 2014 Feb 13;156(4):771-85

TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.

Dibble CC, Elis W, Menon S, Qin W, Klekota J, Asara JM, Finan PM, Kwiatkowski DJ, Murphy LO, Manning BD
Molecular cell 2012 Aug 24;47(4):535-46

Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex.

Boer K, Troost D, Jansen F, Nellist M, van den Ouweland AM, Geurts JJ, Spliet WG, Crino P, Aronica E
Neuropathology : official journal of the Japanese Society of Neuropathology 2008 Dec;28(6):577-90

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Tuberin / TSC2 was done on MCF7 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Tuberin / TSC2 Mouse Monoclonal Antibody (370500, red histogram) or with mouse isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.