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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA324345 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal TSC2 Antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal TSC2 Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TSC2
- Isotype
- IgG
- Antibody clone number
- 249CT19.1.3
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TSC2 Antibody (Cat. #TA324345) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TSC2 antibody detected the TSC2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with TSC2 Antibody(Cat#TA324345/SG110509AA).Hela cells were incubated with TSC2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .TSC2 immunoreactivity is localized to Microtubules significantly.
- Validation comment
- IF
