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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [1]
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- Product number
- PAB8152 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8152, RRID:AB_1716595
- Product name
- TSC2 (phospho S1420) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of TSC2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Biochemical and functional characterizations of small GTPase Rheb and TSC2 GAP activity.
Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent.
Regulation of the TSC pathway by LKB1: evidence of a molecular link between tuberous sclerosis complex and Peutz-Jeghers syndrome.
Li Y, Inoki K, Guan KL
Molecular and cellular biology 2004 Sep;24(18):7965-75
Molecular and cellular biology 2004 Sep;24(18):7965-75
Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent.
Karbowniczek M, Cash T, Cheung M, Robertson GP, Astrinidis A, Henske EP
The Journal of biological chemistry 2004 Jul 16;279(29):29930-7
The Journal of biological chemistry 2004 Jul 16;279(29):29930-7
Regulation of the TSC pathway by LKB1: evidence of a molecular link between tuberous sclerosis complex and Peutz-Jeghers syndrome.
Corradetti MN, Inoki K, Bardeesy N, DePinho RA, Guan KL
Genes & development 2004 Jul 1;18(13):1533-8
Genes & development 2004 Jul 1;18(13):1533-8
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Dot blot analysis of TSC2 (phospho S1420) polyclonal antibody (Cat # PAB8152) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
