NB200-200

antibody from Novus Biologicals

Targeting: TSC2 LAM, PPP1R160, TSC4, tuberin

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-200

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-200, RRID:AB_10003159

Product name

Rabbit Polyclonal TSC2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: TSC2 Antibody [NB200-200] - Detection of human TSC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TSC2 antibody NB200-200 used for IP at 3 ug per reaction. TSC2 was also immunoprecipitated by another rabbit anti-TSC2 antibody. For blotting immunoprecipitated TSC2, NB200-200 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: TSC2 Antibody [NB200-200] - Immunoprecipitation of Human TSC2 and Co-Immunoprecipitation of Human TSC1. Samples: Whole cell lysate (1 mg/IP; 1/4 of IP reaction loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TSC2 antibody (Cat. No. NB200-200) used at 3 mcg.mg lysate for IP. TSC2 was also immunoprecipitated using rabbit anti-TSC2 antibody NB100-626 at 3 mcg/ml. TSC2 and TSC1 in immunoprecipitates were blotted using rabbit anti-TSC2 antibody NB100-626 (0.1 mcg/ml) and rabbit anti-TSC1 antibody NB100-626 (0.1 mcg/ml), respectively. Detection: Chemiluminescence with exposure times of 60 seconds (A) and 30 seconds (B).