Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX116012 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116012, RRID:AB_10626126
- Product name
- Involucrin antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the Inflammatory/Repair Response of Human Keratinocytes.
LncRNA expression profile of ΔNp63α in cervical squamous cancers and its suppressive effects on LIF expression.
Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.
Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells.
Colombo I, Sangiovanni E, Maggio R, Mattozzi C, Zava S, Corbett Y, Fumagalli M, Carlino C, Corsetto PA, Scaccabarozzi D, Calvieri S, Gismondi A, Taramelli D, Dell'Agli M
Mediators of inflammation 2017;2017:7435621
Mediators of inflammation 2017;2017:7435621
LncRNA expression profile of ΔNp63α in cervical squamous cancers and its suppressive effects on LIF expression.
Qian L, Xu F, Wang X, Jiang M, Wang J, Song W, Wu D, Shen Z, Feng D, Ling B, Cheng Y, Xiao W, Shan G, Zhou Y
Cytokine 2017 Aug;96:114-122
Cytokine 2017 Aug;96:114-122
Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
Dang NN, Pang SG, Song HY, An LG, Ma XL
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2015 Jan;48(1):39-45
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2015 Jan;48(1):39-45
Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.
Dang N, Pang S, Song H, Bian H, Zhang X, An L, Ma X
Molecular biology reports 2015 Feb;42(2):337-43
Molecular biology reports 2015 Feb;42(2):337-43
Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells.
Igawa K, Kokubu C, Yusa K, Horie K, Yoshimura Y, Yamauchi K, Suemori H, Yokozeki H, Toyoda M, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Umezawa A, Katayama I, Takeda J
Stem cells translational medicine 2014 Sep;3(9):992-1001
Stem cells translational medicine 2014 Sep;3(9):992-1001
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE Involucrin antibody GTX116012 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using Involucrin(GTX116012) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Involucrin(GTX116012) antibody at 1:250 dilution.