NBP1-05203

antibody from Novus Biologicals

Targeting: MBP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-05203

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-05203, RRID:AB_1556319

Product name

Mouse Monoclonal MBP Antibody

Antibody type

Monoclonal

Description

Affinity purified. The MBP Antibody (7G7) antibody binds all four of the CNS MBP isoforms, so that the epitope for the antibody is located in the core shared by all four gene products.

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Netrin-1 Contributes to Myelinated Afferent Fiber Sprouting and Neuropathic Pain.

Wu CH, Yuan XC, Gao F, Li HP, Cao J, Liu YS, Yu W, Tian B, Meng XF, Shi J, Pan HL, Li M
Molecular neurobiology 2016 Oct;53(8):5640-51

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Phasic Treatment with Interferon Gamma Stimulates Release of Exosomes that Protect Against Spreading Depression.

Pusic AD, Kraig RP
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2015 Oct;35(10):795-807

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Spreading depression transiently disrupts myelin via interferon-gamma signaling.

Pusic AD, Mitchell HM, Kunkler PE, Klauer N, Kraig RP
Experimental neurology 2015 Feb;264:43-54

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MBP Antibody (7G7) [NBP1-05203] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05203, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: MBP Antibody (7G7) [NBP1-05203] - Analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), NBP1-05203, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H, dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.