PA1-18324

antibody from Invitrogen Antibodies

Targeting: MBP

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-18324

Provider

Invitrogen Antibodies

Product name

MBP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Reactivity

Human, Rat, Guinea Pig

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Pannexin 1 Modulates Axonal Growth in Mouse Peripheral Nerves.

Horton SM, Luna Lopez C, Blevins E, Howarth H, Weisberg J, Shestopalov VI, Makarenkova HP, Shah SB
Frontiers in cellular neuroscience 2017;11:365

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Fingerprint changes in CSF composition associated with different aetiologies in human neonatal hydrocephalus: glial proteins associated with cell damage and loss.

Naureen I, Waheed KA, Rathore AW, Victor S, Mallucci C, Goodden JR, Chohan SN, Miyan JA
Fluids and barriers of the CNS 2013 Dec 18;10(1):34

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Western blot analyses for myelin basic protein (MBP). a . Western blots for MBP . Ten-lane precast gels were used. Each lane had an individual 5 mul sample of CSF from the categories of patients shown. After protein separation using SDS PAGE they were blotted onto a PVDF membrane for probing with antibodies. ECL detection was used to visualise the labelled bands and for semi-quantitative analysis using Image-J software. A single 40 KDa band was labelled using the antibody to myelin basic protein and only detected in PHH and SB/HC samples as shown in this representative gel. All samples were run a minimum of three times with a total number of samples as given for semi-quantitative analysis. Patient groups as for Figure 1 . b . Semi-quantitative analysis of the 40 KDa band labelled with anti-MBP antibody. MBP was increased significantly ( P < 0.001) in PHH and SB/HC compared to undetectable levels in normal, FOH and LOH infants. Values are Mean +- SEM of Normal n = 4, FOH n = 4, LOH n = 4, PHH n = 4, SB/HC n = 4. Values in PHH are SB/HC are significantly different to the undetectable level in normal, so effectively from the zero protein level, P < 0.001 indicated by asterisks ***.