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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [5]
- Immunohistochemistry [9]
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- Product number
- R30229 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MBP Antibody / Myelin Basic Protein
- Antibody type
- Polyclonal
- Description
- This highly specific MBP antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with mouse and rat samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the MBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MBP antibody and mouse brain tissue lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MBP antibody and mouse brain tissue lysate. Multiple isoforms may be visualized from 20~37 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MBP antibody with 1) mouse brain and 2) rat brain lysate. Multiple isoforms may be visualized from 20~37 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE mouse brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat brain with MBP antibody (red), anti-GFAP mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of rat brain tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of rat brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of mouse hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of mouse cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of mouse brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of mouse cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of rat hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of rat cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MBP antibody testing of rat cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
