Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-64789 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64789, RRID:AB_964400
- Product name
- Mouse Monoclonal MBP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes myelin basic protein that is phosphorylated on threonine 98.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Testosterone promotes vascular endothelial cell migration via upregulation of ROCK-2/moesin cascade.
Liao W, Huang W, Guo Y, Xin M, Fu X
Molecular biology reports 2013 Dec;40(12):6729-35
Molecular biology reports 2013 Dec;40(12):6729-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MBP [p Thr98] Antibody (98P/12) [NB100-64789] - 30 ug of human brain tissue lysate was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1, 3, 5 and 7. Mouse anti MBP P-Thr98 antibody was used as the primary antibody at a dilution of 1/500 in lanes 2 and 6 and at 1/1000 in lanes 4 and 8. TidyBlot Western Blot Detection Reagent:HRP was used as the secondary reagent at 1/200. Membranes were treated with (+) and without (-) phosphatase and visualized.