MA5-15922

antibody from Invitrogen Antibodies

Targeting: MBP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15922

Provider

Invitrogen Antibodies

Product name

MBP Monoclonal Antibody (2H9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15922 targets MBP in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15922 immunogen is purified recombinant fragment of human MBP expressed in E. Coli. . MA5-15922 detects MBP which has a predicted molecular weight of approximately 33kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2H9

Vial size

100 µL

Concentration

Conc. not determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lasmiditan and 5-Hydroxytryptamine in the rat trigeminal system; expression, release and interactions with 5-HT(1) receptors.

Edvinsson JCA, Maddahi A, Christiansen IM, Reducha PV, Warfvinge K, Sheykhzade M, Edvinsson L, Haanes KA
The journal of headache and pain 2022 Feb 17;23(1):26

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Metformin exacerbates and simvastatin attenuates myelin damage in high fat diet-fed C57BL/6 J mice.

Ciric D, Martinovic T, Petricevic S, Trajkovic V, Bumbasirevic V, Kravic-Stevovic T
Neuropathology : official journal of the Japanese Society of Neuropathology 2018 Oct;38(5):468-474

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MBP using MBP monoclonal antibody (Product # MA5-15922) in HEK293 (1) and MBP-human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MBP using a MBP monoclonal antibody (Product # MA5-15922) against a human MBP (AA: 1-197) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using MBP Monoclonal Antibody (2H9) (Product # MA5-15922) and a 18-20 kDa band corresponding to MBP was observed across tissues tested. Immuno-depleted tissue extracts (30 µg lysate) of Rat Brain (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Brain (Lane 3), Mouse Liver (Lane 4), Mouse Lung (Lane 5), Mouse Skeletal Muscle (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB33001) by iBlot™ 3 Western Blot Transfer Device (Product # IB31001). The blot was probed with the primary antibody (1:2,000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). A 25 kDa band corresponding to IgG light chain was observed in mouse tissue lysates.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MSCS cells using MBP monoclonal antibody (Product # MA5-15922) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MSCS cells using MBP monoclonal antibody (Product # MA5-15922) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded brain tissues using MBP monoclonal antibody (Product # MA5-15922) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cerebellum tissues using MBP monoclonal antibody (Product # MA5-15922) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using MBP monoclonal antibody (Product # MA5-15922) (green) and negative control (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using MBP monoclonal antibody (Product # MA5-15922) (green) and negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Representative confocal lazer microscopic images of double labeled immunofluorescence-stained sciatic nerve sections for MBP (green) and LC3 (red). White arrow indicates yellow colocalization of MBP and LC3.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of 5-HT in relation to myelin basic protein (MBP), SP and CGRP. A MBP showed a distinct staining of myelin sheath flanking cross-sectioned Adelta-fibre axons. A robust MBP-ir was observed (Arrowhead) between the Adelta-fibre axon and Schwann cell neurolemma. An irregular, and sometimes faint, expression of 5-HT could be observed in Adelta-fibre axons (Asterix) and just outside the myelin sheath (Arrow), indicative for the neurolemma of the Schwann cell containing its cytoplasm. B A minority of observed C-fibres expressed 5-HT-ir, these were more frequently found in proximity to Redlich-Obersteiner's zone. CGRP-ir was more abundantly expressed in C-fibres and did not co-localize with 5-HT. Arrows mark 5-HT positive C-fibre boutons and arrowheads mark CGRP positive C-fibre boutons. Insert: Two thin C-fibres entwining and differentially expressing either CGRP or 5-HT. C Similarly, SP could be found concentrated to C-fibre boutons (Arrowhead) throughout the TG. The few 5-HT positive C-fibres (Arrow) were not found to co-localize with SP positive C-fibres. Insert: A thin C-fibre expressing 5-HT-ir (Arrow) above a seemingly larger SP positive C-fibre (Arrowhead)