PA1050

antibody from Boster Biological Technology

Targeting: MBP

Provider product page for PA1050

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [47]
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Validations
Western blot [1]

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Product number: PA1050 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-Myelin Basic Protein/MBP Antibody
Antibody type: Polyclonal
Description: Polyclonal antibody for MBP detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IF. Reactive species: Human. MBP information: Molecular Weight: 33117 MW; Subcellular Localization: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic side of myelin; Tissue Specificity: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

MBP protein structure - PA1050 shown in red.

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Main image
Experimental details: Western blot analysis of MBP using anti-MBP antibody (PA1050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Mouse Brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBP antigen affinity purified polyclonal antibody (Catalog # PA1050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Additional image