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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX48806 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48806, RRID:AB_11179274
- Product name
- NRG1 antibody [k1G13]
- Antibody type
- Monoclonal
- Host
- Mouse
- Vial size
- 100 µl
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot showing detection of 0.1 µg of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein (arrow; expected MW: 63.1 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
- Validation comment
- WB
