PA5-13204

antibody from Invitrogen Antibodies

Targeting: NRG1 GGF, HGL, HRG, NDF, NRG1-IT2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13204

Provider

Invitrogen Antibodies

Product name

NRG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lung injury in axolotl salamanders induces an organ-wide proliferation response.

Jensen TB, Giunta P, Schultz NG, Griffiths JM, Duerr TJ, Kyeremateng Y, Wong H, Adesina A, Monaghan JR
Developmental dynamics : an official publication of the American Association of Anatomists 2021 Jun;250(6):866-879

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Targeting HER2 in patient-derived xenograft ovarian cancer models sensitizes tumors to chemotherapy.

Harris FR, Zhang P, Yang L, Hou X, Leventakos K, Weroha SJ, Vasmatzis G, Kovtun IV
Molecular oncology 2019 Feb;13(2):132-152

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a NRG1 polyclonal antibody (Product # PA5-13204) in SK-BR-3 cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-NRG1 Polyclonal Antibody (Product # PA5-13204) and a 60 kDa band corresponding to NRG1 was observed across the cell lines tested. Whole cell extracts (30ug) of SK-BR-3 (Lane 1), MCF7 (Lane 2), T47D (Lane 3), MDA-MB-231 (Lane 4), U-87 MG (Lane 5) and A-375 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NCI-H460 cells using a NRG1 polyclonal antibody (Product # PA5-13204) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4 FIGURE Expression of genes associated with Nrg1/ErbB signaling. (A) HCR FISH for nrg1 in the IX/X cranial nerve and in the spinal cord. Scale bar = 500 um. Inset = 100 um. (B) Immunohistochemistry of Nrg1 and acetylated beta-Tubulin staining cell bodies and axons of peripheral nerves. Scale bar = 100 um. (C-C') Immunohistochemistry of Nrg1 in the injured lung 21 dpa. Notice expression in ciliated cells and smooth muscle cells. (C'') No primary control immunohistochemical stain. Scale bar in C = 250 um. Scale bar in C' = 50 um. Scale bar in C'' = 100 um. (D) HCR FISH of nrg1 and EdU staining in the injured lung 21 dpa. Notice proliferation in both epithelial and mesenchymal cell types. (E) Heat map of qPCR fold increases at both 7 dpa (n = 3) and 21 dpa (n = 4) for the left (injured) and right (contralateral) lungs. Red asterisks denote significant upregulation of mRNA products ( P < 0.05), while orange asterisks denote trending toward significance ( P < 0.10). (E') Table of significant fold changes ( P < 0.05) for the qPCR results with fold changes indicated. IX/X, IX/X cranial nerve ganglion; o, optic cup; sc, spinal cord