Antibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [6]
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- Product number
- MA5-12896 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NRG1 Monoclonal Antibody (7D5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-12896 targets Heregulin in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human, mouse, and Rat samples.
- Antibody clone number
- 7D5
- Concentration
- 0.2 mg/mL
Submitted references The laterodorsal tegmentum-ventral tegmental area circuit controls depression-like behaviors by activating ErbB4 in DA neurons.
Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma.
Nedd4l downregulation of NRG1 in the mPFC induces depression-like behaviour in CSDS mice.
Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma.
A novel role for cilia-dependent sonic hedgehog signaling during submandibular gland development.
Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model.
Expression and Localization of Neuregulin-1 (Nrg1) and ErbB2/ErbB4 Receptors in Main Endocrine Organs of the Rhesus Monkey.
Endogenous expression of Neuregulin-1 (Nrg1) as a potential modulator of prolactin (PRL) secretion in GH3 cells.
Endothelium-derived neuregulin protects the heart against ischemic injury.
Activation of the neuregulin/ErbB system during physiological ventricular remodeling in pregnancy.
Neuregulin-1-human epidermal receptor-2 signaling is a central regulator of pulmonary epithelial permeability and acute lung injury.
Neuregulin-1 (Nrg1) is mainly expressed in rat pituitary gonadotroph cells and possibly regulates prolactin (PRL) secretion in a juxtacrine manner.
Metalloproteinase-dependent cleavage of neuregulin and autocrine stimulation of vascular endothelial cells.
Phenotypic characterization of transgenic mice overexpressing neuregulin-1.
Ventricular ErbB2/ErbB4 activation and downstream signaling in pacing-induced heart failure.
Neuregulin-1 signaling in brain endothelial cells.
Peripheral neuropathy in the twitcher mouse: accumulation of extracellular matrix in the endoneurium and aberrant expression of ion channels.
Heregulin, cysteine rich-61 and matrix metalloproteinase 9 expression in human carotid atherosclerotic plaques: relationship with clinical data.
Localization of neuregulin-1alpha (heregulin-alpha) and one of its receptors, ErbB-4 tyrosine kinase, in developing and adult human brain.
Role of neuregulin-1/ErbB2 signaling in endothelium-cardiomyocyte cross-talk.
Constitutive neuregulin-1/ErbB signaling contributes to human vestibular schwannoma proliferation.
Expression of heregulin in human coronary atherosclerotic lesions.
NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis.
Paclitaxel and carboplatin as first-line chemotherapy combined with gefitinib (IRESSA) in patients with advanced breast cancer: a phase I/II study conducted by the Hellenic Cooperative Oncology Group.
Neuregulin-1 enhances survival of human astrocytic glioma cells.
Tissue interaction mediated by neuregulin-1 and ErbB receptors regulates epithelial morphogenesis of mouse embryonic submandibular gland.
Back signaling by the Nrg-1 intracellular domain.
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
Wang H, Cui W, Chen W, Liu F, Dong Z, Xing G, Luo B, Gao N, Zou WJ, Zhao K, Zhang H, Ren X, Yu Z, Robinson HL, Liu Z, Xiong WC, Mei L
Molecular psychiatry 2023 Mar;28(3):1027-1045
Molecular psychiatry 2023 Mar;28(3):1027-1045
Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma.
Lin WW, Ou GY, Lin JZ, Yi SJ, Yao WC, Pan HC, Zhao WJ
Oncology letters 2020 Jul;20(1):326-336
Oncology letters 2020 Jul;20(1):326-336
Nedd4l downregulation of NRG1 in the mPFC induces depression-like behaviour in CSDS mice.
Xu J, Guo C, Liu Y, Wu G, Ke D, Wang Q, Mao J, Wang JZ, Liu R, Wang X
Translational psychiatry 2020 Jul 23;10(1):249
Translational psychiatry 2020 Jul 23;10(1):249
Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma.
Aldaregia J, Errarte P, Olazagoitia-Garmendia A, Gimeno M, Uriz JJ, Gershon TR, Garcia I, Matheu A
Cancers 2020 Apr 17;12(4)
Cancers 2020 Apr 17;12(4)
A novel role for cilia-dependent sonic hedgehog signaling during submandibular gland development.
Elliott KH, Millington G, Brugmann SA
Developmental dynamics : an official publication of the American Association of Anatomists 2018 Jun;247(6):818-831
Developmental dynamics : an official publication of the American Association of Anatomists 2018 Jun;247(6):818-831
Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model.
Yang Z, Jiang Q, Chen SX, Hu CL, Shen HF, Huang PZ, Xu JP, Mei JP, Zhang BP, Zhao WJ
Molecular medicine reports 2016 Jul;14(1):790-6
Molecular medicine reports 2016 Jul;14(1):790-6
Expression and Localization of Neuregulin-1 (Nrg1) and ErbB2/ErbB4 Receptors in Main Endocrine Organs of the Rhesus Monkey.
Zhao WJ
International journal of endocrinology and metabolism 2013 Summer;11(3):162-6
International journal of endocrinology and metabolism 2013 Summer;11(3):162-6
Endogenous expression of Neuregulin-1 (Nrg1) as a potential modulator of prolactin (PRL) secretion in GH3 cells.
Zhao W, Shen Y, Ren S
Cell and tissue research 2011 May;344(2):313-20
Cell and tissue research 2011 May;344(2):313-20
Endothelium-derived neuregulin protects the heart against ischemic injury.
Hedhli N, Huang Q, Kalinowski A, Palmeri M, Hu X, Russell RR, Russell KS
Circulation 2011 May 24;123(20):2254-62
Circulation 2011 May 24;123(20):2254-62
Activation of the neuregulin/ErbB system during physiological ventricular remodeling in pregnancy.
Lemmens K, Doggen K, De Keulenaer GW
American journal of physiology. Heart and circulatory physiology 2011 Mar;300(3):H931-42
American journal of physiology. Heart and circulatory physiology 2011 Mar;300(3):H931-42
Neuregulin-1-human epidermal receptor-2 signaling is a central regulator of pulmonary epithelial permeability and acute lung injury.
Finigan JH, Faress JA, Wilkinson E, Mishra RS, Nethery DE, Wyler D, Shatat M, Ware LB, Matthay MA, Mason R, Silver RF, Kern JA
The Journal of biological chemistry 2011 Mar 25;286(12):10660-70
The Journal of biological chemistry 2011 Mar 25;286(12):10660-70
Neuregulin-1 (Nrg1) is mainly expressed in rat pituitary gonadotroph cells and possibly regulates prolactin (PRL) secretion in a juxtacrine manner.
Zhao W, Ren SG
Journal of neuroendocrinology 2011 Dec;23(12):1252-62
Journal of neuroendocrinology 2011 Dec;23(12):1252-62
Metalloproteinase-dependent cleavage of neuregulin and autocrine stimulation of vascular endothelial cells.
Kalinowski A, Plowes NJ, Huang Q, Berdejo-Izquierdo C, Russell RR, Russell KS
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jul;24(7):2567-75
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jul;24(7):2567-75
Phenotypic characterization of transgenic mice overexpressing neuregulin-1.
Kato T, Kasai A, Mizuno M, Fengyi L, Shintani N, Maeda S, Yokoyama M, Ozaki M, Nawa H
PloS one 2010 Dec 9;5(12):e14185
PloS one 2010 Dec 9;5(12):e14185
Ventricular ErbB2/ErbB4 activation and downstream signaling in pacing-induced heart failure.
Doggen K, Ray L, Mathieu M, Mc Entee K, Lemmens K, De Keulenaer GW
Journal of molecular and cellular cardiology 2009 Jan;46(1):33-8
Journal of molecular and cellular cardiology 2009 Jan;46(1):33-8
Neuregulin-1 signaling in brain endothelial cells.
Lok J, Sardi SP, Guo S, Besancon E, Ha DM, Rosell A, Kim WJ, Corfas G, Lo EH
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2009 Jan;29(1):39-43
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2009 Jan;29(1):39-43
Peripheral neuropathy in the twitcher mouse: accumulation of extracellular matrix in the endoneurium and aberrant expression of ion channels.
Kagitani-Shimono K, Mohri I, Yagi T, Taniike M, Suzuki K
Acta neuropathologica 2008 May;115(5):577-87
Acta neuropathologica 2008 May;115(5):577-87
Heregulin, cysteine rich-61 and matrix metalloproteinase 9 expression in human carotid atherosclerotic plaques: relationship with clinical data.
Sigala F, Georgopoulos S, Papalambros E, Chasiotis D, Vourliotakis G, Niforou A, Kotsinas A, Kavantzas N, Patsouris E, Gorgoulis VG, Bastounis E
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 Sep;32(3):238-45
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 Sep;32(3):238-45
Localization of neuregulin-1alpha (heregulin-alpha) and one of its receptors, ErbB-4 tyrosine kinase, in developing and adult human brain.
Bernstein HG, Lendeckel U, Bertram I, Bukowska A, Kanakis D, Dobrowolny H, Stauch R, Krell D, Mawrin C, Budinger E, Keilhoff G, Bogerts B
Brain research bulletin 2006 May 15;69(5):546-59
Brain research bulletin 2006 May 15;69(5):546-59
Role of neuregulin-1/ErbB2 signaling in endothelium-cardiomyocyte cross-talk.
Lemmens K, Segers VF, Demolder M, De Keulenaer GW
The Journal of biological chemistry 2006 Jul 14;281(28):19469-77
The Journal of biological chemistry 2006 Jul 14;281(28):19469-77
Constitutive neuregulin-1/ErbB signaling contributes to human vestibular schwannoma proliferation.
Hansen MR, Roehm PC, Chatterjee P, Green SH
Glia 2006 Apr 15;53(6):593-600
Glia 2006 Apr 15;53(6):593-600
Expression of heregulin in human coronary atherosclerotic lesions.
Panutsopulos D, Arvanitis DL, Tsatsanis C, Papalambros E, Sigala F, Spandidos DA
Journal of vascular research 2005 Nov-Dec;42(6):463-74
Journal of vascular research 2005 Nov-Dec;42(6):463-74
NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis.
Prentice LM, Shadeo A, Lestou VS, Miller MA, deLeeuw RJ, Makretsov N, Turbin D, Brown LA, Macpherson N, Yorida E, Cheang MC, Bentley J, Chia S, Nielsen TO, Gilks CB, Lam W, Huntsman DG
Oncogene 2005 Nov 10;24(49):7281-9
Oncogene 2005 Nov 10;24(49):7281-9
Paclitaxel and carboplatin as first-line chemotherapy combined with gefitinib (IRESSA) in patients with advanced breast cancer: a phase I/II study conducted by the Hellenic Cooperative Oncology Group.
Fountzilas G, Pectasides D, Kalogera-Fountzila A, Skarlos D, Kalofonos HP, Papadimitriou C, Bafaloukos D, Lambropoulos S, Papadopoulos S, Kourea H, Markopoulos C, Linardou H, Mavroudis D, Briasoulis E, Pavlidis N, Razis E, Kosmidis P, Gogas H
Breast cancer research and treatment 2005 Jul;92(1):1-9
Breast cancer research and treatment 2005 Jul;92(1):1-9
Neuregulin-1 enhances survival of human astrocytic glioma cells.
Ritch PS, Carroll SL, Sontheimer H
Glia 2005 Aug 15;51(3):217-28
Glia 2005 Aug 15;51(3):217-28
Tissue interaction mediated by neuregulin-1 and ErbB receptors regulates epithelial morphogenesis of mouse embryonic submandibular gland.
Miyazaki Y, Nakanishi Y, Hieda Y
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Aug;230(4):591-6
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Aug;230(4):591-6
Back signaling by the Nrg-1 intracellular domain.
Bao J, Wolpowitz D, Role LW, Talmage DA
The Journal of cell biology 2003 Jun 23;161(6):1133-41
The Journal of cell biology 2003 Jun 23;161(6):1133-41
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
Hansen MR, Vijapurkar U, Koland JG, Green SH
Hearing research 2001 Nov;161(1-2):87-98
Hearing research 2001 Nov;161(1-2):87-98
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
Hansen MR, Vijapurkar U, Koland JG, Green SH
Hearing research 2001 Nov;161(1-2):87-98
Hearing research 2001 Nov;161(1-2):87-98
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot of Heregulin using Heregulin Monoclonal Antibody (Product # MA5-12896) on HRGa1 Cells and HRGb1 Cells.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Immunofluorescence analysis of NRG1 was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NRG1 (7D5) Mouse Monoclonal Antibody (Product # MA5-12896) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous, cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
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- Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Heregulin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.
Supportive validation
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- Invitrogen Antibodies (provider)
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- NULL
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- Invitrogen Antibodies (provider)
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- Fig. 1 CSDS induces depression accompanied by an increase in NRG1 degradation. a Experimental timeline of CSDS, SI, behavioural studies and tissue collection. CSDS-induced depression-like behaviours as assessed by b SI test, c the sucrose preference test and d the tail suspension test, and anxiety-like behaviours as assessed by e novelty-suppressed feeding, f the open field test and g the elevated plus maze test, n = 12/12/8. h , i Golgi staining was performed to evaluate changes in dendritic spines in the mPFC between CTR, SS and RES mice, and at least ten neurons from three mice per group were analysed. Scale bars, 10 mum. j - l NRG1 protein level ( n = 8/8/4) was lower in the mPFC of SS mice. There were no differences in NRG1 mRNA levels ( n = 9/8/6) between CTR, SS and RES mice. m - o Experimental timeline of treatment with dexamethasone (Dex), cycloheximide (CHX) and MG132 in primary cortical neurons. Primary cortical neurons were cultured in vitro for a total of 20 days in vitro, including treatment with 0.5 uM Dex twice a day from DIV 10 to DIV 20. Western blot was used to determine levels of NRG1 in primary neurons treated with 10 mug/mL CHX for 4 h, 8 h or 12 h in DIV 20, n = 3 per group. p - r Inhibition of proteasomes by treatment with 10 muM MG132 for 12 h of cultured primary neurons induces NRG1 elevation with Dex treatment for 10 days as measured by western blotting. There were no differences in NRG1 mRNA levels among all groups, n = 3 per group. p -Value signif
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- Fig. 2 Overexpression of NRG1 attenuates CSDS-induced depression-like behaviours. a Experimental timeline of mPFC bilateral injection of AAV9-EGFP or AAV9-OE-NRG1 viruses and behavioural studies. b , c Representative western blots and quantification of NRG1 protein in the mPFC of non-stressed (CTR) mice, and mice subjected to CSDS after bilateral injection of AAV9-EGFP or AAV9-OE-NRG1 viruses, n = 3 per group. Overexpression of NRG1 before CSDS-induced depression-like behaviours as assessed by d social interaction, e sucrose preference, f novelty-suppressed feeding, g tail suspension test, h open field test and i the elevated plus maze test, n = 10/7/10/8. p -Value significance is calculated from a one-way ANOVA or two-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. All data represent mean +- SEM.
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- Fig. 3 The interaction between Nedd4l and NRG1 promotes the ubiquitination and degradation of NRG1. a Scatter plot of KEGG pathway enrichment from RNA-seq data showed significant changes related to protein degradation in the CTR and SS mice. Highlighted by the red box is the ubiquitin-mediated proteolysis pathway, in which we were most interested. b - d Mice subjected to CSDS showed elevated Nedd4l protein levels in the medial prefrontal cortex (mPFC) of SS mice as measured by western blotting, n = 6 per group. The mRNA level of Nedd4l was enhanced in the mPFC samples of SS mice, and the same result was found with RNA-seq, n = 4/5/3. e - g The mPFC tissue homogenates were immunoprecipitated (IP), using anti-NRG1 and anti-Nedd4l antibodies, n = 4 per group; NRG1 ubiquitination was analysed by western blotting and quantification in CTR, SS and RES mice, n = 3 per group. h - k Representative western blots and quantification of NRG1, Nedd4l and NRG1 ubiquitination (UBI) identified in primary cortical neurons cultured in vitro for a total of 20 days, including treatment with 0.5 uM Dex from DIV 10 to DIV 20, n = 4 per group, two-tailed Student's t -test. l - n Representative western blots and quantification of NRG1 and Nedd4l identified in primary cortical neurons cultured for 20 days, and treated with 1 µL/mL LV-Nedd4l-RNAi virus after treatment with Dex, n = 3 per group. p -Value significance is calculated from a one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. All d
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- Fig. 4 Overexpression of Nedd4l deteriorates subthreshold social defeat stress-induced depression-like behaviours. a Experimental timeline of mPFC bilateral injection of AAV9-EGFP or AAV9-OE-Nedd4l viruses and behavioural studies. Overexpression of Nedd4l before subthreshold social defeat stress (SSDS)-induced depression-like behaviours as assessed by b social interaction, c sucrose preference test and d tail suspension test, and anxiety-like behaviours as assessed by e novelty-suppressed feeding test, f open field test and g the elevated plus maze test, n = 20/9/18/22. h - k Representative western blots and quantification of NRG1, Nedd4l and NRG1 ubiquitination (UBI) identified in the mPFC of non-stressed (CTR) mice, and mice subjected to SSDS after bilateral injection of AAV9-EGFP or AAV9-OE-Nedd4l viruses, n = 3-4 per group. l , m Golgi staining was performed to evaluate the alterations of dendritic spines in the mPFC between CTR and SSDS mice after injection of AAV9-EGFP or AAV9-OE-Nedd4l viruses. At least ten neurons from three mice per group were analysed. Scale bars, 10 mum. p -Value significance is calculated from a one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. All data represent mean +- SEM.
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- Fig. 5 Knock down of Nedd4l rescues CSDS-induced depression. a Experimental timeline of mPFC bilateral injection of AAV9-EGFP or AAV9-Sh-Nedd4l viruses and behavioural studies. Downregulation of Nedd4l expression before CSDS-induced depression-like behaviours as assessed by b social interaction, c sucrose preference and d tail suspension tests, and anxiety-like behaviours as assessed by e novelty-suppressed feeding, f open field and g the elevated plus maze tests, n = 10/8/8/8. h - k Representative western blots and quantification of NRG1, Nedd4l and NRG1 ubiquitination (UBI) identified in the mPFC of non-stressed (CTR) mice, and mice subjected to CSDS after bilateral injection of AAV9-EGFP or AAV9-Sh-Nedd4l viruses, n = 3 per group. l , m Golgi staining was performed to evaluate the changes of dendritic spines in the mPFC between CTR and CSDS mice after injection of AAV9-EGFP or AAV9-Sh-Nedd4l viruses. At least ten neurons from three mice per group were analysed. Scale bars, 10 mum. p -Value significance is calculated from a one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. All data represent mean +- SEM.