Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- PA5-27331 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NRG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Mouse brain, Rat brain. Predicted reactivity: Mouse (91%), Rat (91%), Chicken (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Neuregulin-1 in Rat tissue extract (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Neuregulin-1 Polyclonal antibody (Product # PA5-27331) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Neuregulin-1 in Mouse tissue extract (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Neuregulin-1 Polyclonal antibody (Product # PA5-27331) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NRG1 Polyclonal Antibody (Product # PA5-27331). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. NRG1 Polyclonal Antibody (Product # PA5-27331) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NRG1 Polyclonal Antibody (Product # PA5-27331). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NRG1 Polyclonal Antibody (Product # PA5-27331) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NRG1 Polyclonal Antibody (Product # PA5-27331). Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NRG1 Polyclonal Antibody (Product # PA5-27331) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NRG1 Polyclonal Antibody detects Neuregulin-1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuregulin-1 stained by NRG1 Polyclonal Antibody (Product # PA5-27331) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .