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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF378 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NRG1 Isoform SMDF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human NRG1 Isoform SMDF in direct ELISAs and Western blots. Neutralizes 60-80% of the biological activity of human NRG1 Isoform SMDF.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_039253- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by NRG1 Isoform SMDF and Neutralization by Human NRG1 Isoform SMDF Anti-body. Recombinant Human NRG1 Isoform SMDF (Catalog # 378-SM) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1 Isoform SMDF (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1 Isoform SMDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF378). The ND50 is typically 2-8 µg/mL.
