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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-396-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human NRG1-beta 1/HRG1-beta 1 EGF Domain in direct ELISAs and Western blots. In Western blots (reducing conditions), approximately 100% cross-reactivity with recombinant human HRG-alpha is observed. In direct ELISAs, approximately 5% cross-reactivity with recombinant human HRG-alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_039250- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
Circulation research 2009 Aug 28;105(5):500-10
Circulation research 2009 Aug 28;105(5):500-10
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NRG1-beta 1/HRG1-beta 1 in Human Breast . NRG1-beta 1/HRG1-beta 1 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-396-NA) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by NRG1-beta 1/HRG1-beta 1 and Neutralization by Human NRG1-beta 1/HRG1-beta 1 Antibody. Recombinant Human NRG1-beta 1/ HRG1-beta 1 EGF Domain (Catalog # 396-HB) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1-beta 1/ HRG1-beta 1 EGF Domain (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-396-NA). The ND50 is typically 0.5-2.0 µg/mL.
