Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47892 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FABP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-human/mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47892) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FABP1 in human liver tissue, mouse liver tissue, and rat liver tissue. Samples were incubated in FABP1 polyclonal antibody (Product # PA5-47892) using a dilution of 0.1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for FABP1/L‚FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FABP1 in 0.2 mg/mL lysates of human liver tissue. Samples were incubated in FABP1 polyclonal antibody (Product # PA5-47892) using a dilution of 1 µg/mL followed by HRP-conjugated Anti-Sheep IgG at a dilution of 1:50. A specific band was detected for FABP1/L‚FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FABP1 in perfusion fixed frozen sections of mouse intestine. Samples were incubated in FABP1 polyclonal antibody (Product # PA5-47892) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical portion of epithelial cells.