Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB29643-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat FABP1/L-FABP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and rat FABP1/L-FABP in direct ELISAs and Western blots. In sandwich immunoassays, it is specific for human FABP1/L-FABP.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P07148- Isotype
- IgG
- Antibody clone number
- 965608
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat FABP1/L-FABP by Simple WesternTM. Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29643) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat FABP1/L-FABP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, Hep3B human hepatocellular carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
