AF2964

antibody from R&D Systems

Targeting: FABP1 L-FABP

Antibody data

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Validation data

Product number: AF2964 - Provider product page
Provider: R&D Systems
Product name: Human/Mouse/Rat FABP1/L-FABP Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human FABP1/L-FABP in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human FABP2, 3, 4, 5, 7, and recombinant mouse FABP9 is observed.
Reactivity: Human, Mouse, Rat
Host: Goat
Conjugate: Unconjugated
Antigen sequence: P07148
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

FABP1 protein structure - AF2964 shown in red.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human, Mouse, and Rat FABP1/L-FABP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human FABP1/L-FABP by Western Blot. Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/ Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human FABP1/L-FABP by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human and Mouse FABP1/L-FABP by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 14-16 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.