10351-1-AP

antibody from Proteintech Group

Targeting: EMD LEMD5, STA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10351-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10351-1-AP, RRID:AB_2100056

Product name

Emerin antibody

Antibody type

Polyclonal

Description

Emerin antibody (Cat. #10351-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IP, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Emerin expression stratification across breast cancer subtypes.

Ghiarone T, Hansen E, Holaska JM
Cancer treatment and research communications 2025;45:101037

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Novel LBR pathogenic variants with loss of sterol reductase activity participate in the pathogenesis of skeletal dysplasia via dysregulating canonical Wnt pathway.

Chen Y, Bai Y, Deng D, Liu Y, He L, Wang M, Zhou M, Wang X, Yu C, Wang Y, Zhao F, Hai C, Wang B, Liu J, Kong X, Tu X, Li H
Biochimica et biophysica acta. Molecular basis of disease 2025 Oct;1871(7):167901

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ZBP1 senses splicing aberration through Z-RNA to promote cell death.

Yang ZH, Wu P, Zhang BX, Yang CR, Huang J, Wu L, Guo SH, Zhou Y, Mao Y, Yin Y, Wu X, Cheng P, Li B, Zhou R, Shen HM, Nie S, Cai ZY, Mo W
Molecular cell 2025 May 1;85(9):1775-1789.e5

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Emerin expression stratification across breast cancer subtypes.

Ghiarone T, Hansen E, Holaska JM
bioRxiv : the preprint server for biology 2025 Jul 7;

---

Tail Anchored protein insertion mediated by CAML and TRC40 links to neuromuscular function in mice.

Zhang Y, He L, Gundelach J, Ge A, Edlund H, Norlin S, Bram RJ
PLoS genetics 2025 Jan;21(1):e1011547

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Connexin 43 as a novel Ca(2+) transport pathway in the nuclear envelope of human induced pluripotent stem cell derived cardiomyocytes.

Toth N, Helassa N, Morad M
Cell calcium 2025 Dec;132:103090

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C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.

Mella J, Volk RF, Zaro BW, Buchwalter A
eLife 2025 Aug 28;14

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Development of Emerin mRNA Lipid Nanoparticles to Rescue Myogenic Differentiation.

Marano N, Guner LE, Riley RS, Holaska JM
International journal of molecular sciences 2025 Aug 12;26(16)

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Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite.

Li Y, Huang M, Wang M, Wang Y, Deng P, Li C, Huang J, Chen H, Wei Z, Ouyang Q, Zhao J, Lu Y, Su S
Cancer cell 2024 Jun 10;42(6):985-1002.e18

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OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools.

Lee A, Sung G, Shin S, Lee SY, Sim J, Nhung TTM, Nghi TD, Park SK, Sathieshkumar PP, Kang I, Mun JY, Kim JS, Rhee HW, Park KM, Kim K
Nature communications 2024 Feb 29;15(1):1851

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Emerin deficiency drives MCF7 cells to an invasive phenotype.

Hansen E, Rolling C, Wang M, Holaska JM
bioRxiv : the preprint server for biology 2024 Aug 7;

---

A p62-dependent rheostat dictates micronuclei catastrophe and chromosome rearrangements.

Martin S, Scorzoni S, Cordone S, Mazzagatti A, Beznoussenko GV, Gunn AL, Di Bona M, Eliezer Y, Leor G, Ben-Yishay T, Loffreda A, Cancila V, Rainone MC, Ippolito MR, Martis V, Bedin F, Garrè M, Vaites LP, Vasapolli P, Polo S, Parazzoli D, Tripodo C, Mironov AA, Cuomo A, Ben-David U, Bakhoum SF, Hatch EM, Ly P, Santaguida S
Science (New York, N.Y.) 2024 Aug 30;385(6712):eadj7446

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Emerin deficiency drives MCF7 cells to an invasive phenotype.

Hansen E, Rolling C, Wang M, Holaska JM
Scientific reports 2024 Aug 28;14(1):19998

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A membrane-sensing mechanism links lipid metabolism to protein degradation at the nuclear envelope.

Lee S, Carrasquillo Rodrı Guez JW, Merta H, Bahmanyar S
The Journal of cell biology 2023 Sep 4;222(9)

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Nuclear lamin A-associated proteins are required for centromere assembly.

Landeros A, Wallace DA, Rahi A, Magdongon CB, Suraneni P, Amin MA, Chakraborty M, Adam SA, Foltz DR, Varma D
bioRxiv : the preprint server for biology 2023 Sep 26;

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Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation.

Sun S, Zheng Z, Wang J, Li F, He A, Lai K, Zhang S, Lu JH, Tian R, Tan CSH
Nature communications 2023 Nov 24;14(1):7697

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Chromatin remodeling and nucleoskeleton synergistically control osteogenic differentiation in different matrix stiffnesses.

Xu X, Zhang H, Li Y, Liu F, Jing Z, Ren M, Chen T, Fu Y, Wu Y, Ji P, Yang S
Materials today. Bio 2023 Jun;20:100661

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LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion.

Jung-Garcia Y, Maiques O, Monger J, Rodriguez-Hernandez I, Fanshawe B, Domart MC, Renshaw MJ, Marti RM, Matias-Guiu X, Collinson LM, Sanz-Moreno V, Carlton JG
Nature cell biology 2023 Jan;25(1):108-119

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Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery.

Marano N, Holaska JM
Frontiers in cell and developmental biology 2022;10:1007120

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The BAF A12T mutation disrupts lamin A/C interaction, impairing robust repair of nuclear envelope ruptures in Nestor-Guillermo progeria syndrome cells.

Janssen A, Marcelot A, Breusegem S, Legrand P, Zinn-Justin S, Larrieu D
Nucleic acids research 2022 Sep 9;50(16):9260-9278

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BCAP31 is involved in modulating colorectal cancer cell proliferation via the Emerin/β-catenin axis.

Han L, Shi J, Zhao L, Deng J, Li Y, Zhao H, Wang H, Yan Y, Zou F
Experimental cell research 2022 Sep 1;418(1):113265

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Recruitment of DNA to tumor-derived microvesicles.

Clancy JW, Sheehan CS, Boomgarden AC, D'Souza-Schorey C
Cell reports 2022 Mar 1;38(9):110443

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Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.

He T, Zhang Y, Li X, Liu C, Zhu G, Yin X, Zhang Z, Zhao K, Wang Z, Zhao P, Wang K
World journal of surgical oncology 2022 Jun 2;20(1):174

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Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.

Golchoubian B, Brunner A, Bragulat-Teixidor H, Neuner A, Akarlar BA, Ozlu N, Schlaitz AL
The Journal of cell biology 2022 Feb 7;221(2)

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Defects in Emerin-Nucleoskeleton Binding Disrupt Nuclear Structure and Promote Breast Cancer Cell Motility and Metastasis.

Liddane AG, McNamara CA, Campbell MC, Mercier I, Holaska JM
Molecular cancer research : MCR 2021 Jul;19(7):1196-1207

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CDK1 controls CHMP7-dependent nuclear envelope reformation.

Gatta AT, Olmos Y, Stoten CL, Chen Q, Rosenthal PB, Carlton JG
eLife 2021 Jul 21;10

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Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.

Merta H, Carrasquillo Rodríguez JW, Anjur-Dietrich MI, Vitale T, Granade ME, Harris TE, Needleman DJ, Bahmanyar S
Developmental cell 2021 Dec 20;56(24):3364-3379.e10

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Off-the-shelf proximity biotinylation for interaction proteomics.

Santos-Barriopedro I, van Mierlo G, Vermeulen M
Nature communications 2021 Aug 18;12(1):5015

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Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Procter DJ, Furey C, Garza-Gongora AG, Kosak ST, Walsh D
Nature 2020 Nov;587(7832):109-114

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EDMD-Causing Emerin Mutant Myogenic Progenitors Exhibit Impaired Differentiation Using Similar Mechanisms.

Iyer A, Holaska JM
Cells 2020 Jun 15;9(6)

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Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients.

Dubińska-Magiera M, Kozioł K, Machowska M, Piekarowicz K, Filipczak D, Rzepecki R
Cells 2019 Mar 13;8(3)

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Targeting of LRRC59 to the Endoplasmic Reticulum and the Inner Nuclear Membrane.

Blenski M, Kehlenbach RH
International journal of molecular sciences 2019 Jan 15;20(2)

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Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Liu S, Kwon M, Mannino M, Yang N, Renda F, Khodjakov A, Pellman D
Nature 2018 Sep;561(7724):551-555

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Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression.

Smith ER, Meng Y, Moore R, Tse JD, Xu AG, Xu XX
BMC cell biology 2017 Jan 14;18(1):8

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The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.

Piekarowicz K, Machowska M, Dratkiewicz E, Lorek D, Madej-Pilarczyk A, Rzepecki R
Chromosoma 2017 Aug;126(4):501-517

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DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.

Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW
Cell 2017 Aug 24;170(5):956-972.e23

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Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Pfaff J, Rivera Monroy J, Jamieson C, Rajanala K, Vilardi F, Schwappach B, Kehlenbach RH
Journal of cell science 2016 Feb 1;129(3):502-16

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Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.

Lenain C, Gusyatiner O, Douma S, van den Broek B, Peeper DS
Carcinogenesis 2015 Nov;36(11):1263-74

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MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.

Fu Y, Lv P, Yan G, Fan H, Cheng L, Zhang F, Dang Y, Wu H, Wen B
Scientific reports 2015 Nov 25;5:17186

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10351-1-AP(EMD antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using EMD antibody 10351-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP result of EMD antibody (10351-1-AP for IP and Detection) with mouse skeletal muscle tissue lysate.

Sample type

tissue