H00002010-M01

antibody from Abnova Corporation

Targeting: EMD LEMD5, STA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002010-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002010-M01, RRID:AB_464358

Product name

EMD monoclonal antibody (M01), clone 3B9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant EMD.

Antigen sequence

MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYE
KKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGD
ADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTY
GEPES


Isotype

IgG

Antibody clone number

3B9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EMD monoclonal antibody (M01), clone 3B9 Western Blot analysis of EMD expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EMD is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to EMD on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to EMD on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol