Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- HPA000609 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA000609, RRID:AB_1078736
- Product name
- Anti-EMD
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human EMD, Gene description: emerin, Alternative Gene Names: LEMD5, STA, Validated applications: WB, IHC, ICC, Uniprot ID: P50402, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Endothelial progerin expression causes cardiovascular pathology through an impaired mechanoresponse
Spindle associated membrane protein 1 (Samp1) is required for the differentiation of muscle cells
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Impact of genomic stability on protein expression in endometrioid endometrial cancer
Osmanagic-Myers S, Kiss A, Manakanatas C, Hamza O, Sedlmayer F, Szabo P, Fischer I, Fichtinger P, Podesser B, Eriksson M, Foisner R
Journal of Clinical Investigation 2018;129(2):531-545
Journal of Clinical Investigation 2018;129(2):531-545
Spindle associated membrane protein 1 (Samp1) is required for the differentiation of muscle cells
Jafferali M, Figueroa R, Hasan M, Hallberg E
Scientific Reports 2017;7(1)
Scientific Reports 2017;7(1)
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
Impact of genomic stability on protein expression in endometrioid endometrial cancer
Lomnytska M, Becker S, Gemoll T, Lundgren C, Habermann J, Olsson A, Bodin I, Engström U, Hellman U, Hellman K, Hellström A, Andersson S, Mints M, Auer G
British Journal of Cancer 2012;106(7):1297-1305
British Journal of Cancer 2012;106(7):1297-1305
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum.
- Sample type
- Human